ArticleScientific reports2016
Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Direct Comparison of MRM-MS and PRM-MS Methods for Quantitative Ganglioside Analysis.Journal of the American Society for Mass Spectrometry · 2026Article
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Structure Analysis of Gangliosides: Mass Spectrometry Imaging.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Glycosphingolipids in Dementia: Insights from Mass Spectrometry and Systems Biology Approaches.Biomedicines · 2025Review
- Establishment of a targeted analysis method for gangliosides in mouse tissues by HILIC-ESI-MS/MS.Analytical and bioanalytical chemistry · 2024Article
- MALDI TIMS IMS Reveals Ganglioside Molecular Diversity within MurineJournal of the American Society for Mass Spectrometry · 2024Article
- Frontiers in Mass Spectrometry-Based Spatial Metabolomics: Current Applications and Challenges in the Context of Biomedical Research.Trends in analytical chemistry : TRAC · 2024Article
- Hepatic Topology of Glycosphingolipids inAnalytical chemistry · 2024Article
- Advances in Mass Spectrometry of Gangliosides Expressed in Brain Cancers.International journal of molecular sciences · 2024Review
- Mass Spectrometry-Based Analysis of Lipid Involvement in Alzheimer's Disease Pathology-A Review.Metabolites · 2022Review
- Proteomic Approaches to Unravel Mechanisms of Antibiotic Resistance and Immune Evasion of Bacterial Pathogens.Frontiers in medicine · 2022Review
- Manipulation of Ion Types via Gas-Phase Ion/Ion Chemistry for the Structural Characterization of the Glycan Moiety on Gangliosides.Analytical chemistry · 2021Article
- Streamlined Multimodal DESI and MALDI Mass Spectrometry Imaging on a Singular Dual-Source FT-ICR Mass Spectrometer.Metabolites · 2021Article
- Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging.STAR protocols · 2021Article
- Mass Spectrometry Imaging for Glycome in the Brain.Frontiers in neuroanatomy · 2021Review
- Recent advances in the mass spectrometric analysis of glycosphingolipidome - A review.Analytica chimica acta · 2020Review
- Lipid rafts and neurodegeneration: structural and functional roles in physiologic aging and neurodegenerative diseases.Journal of lipid research · 2020Review
- Soft Matrix-Assisted Laser Desorption/Ionization for Labile Glycoconjugates.Journal of the American Society for Mass Spectrometry · 2019Article
- MALDI imaging delineates hippocampal glycosphingolipid changes associated with neurotoxin induced proteopathy following neonatal BMAA exposure.Biochimica et biophysica acta. Proteins and proteomics · 2017Article
- Improved MALDI imaging MS analysis of phospholipids using graphene oxide as new matrix.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gangliosides are a family of complex lipids that are abundant in the brain. There is no doubt the investigations about the distribution of gangliosides in brian and the relationship between gangliosides and Alzheimer's disease is profound. However, these investigations are full of challenges due to the structural complexity of gangliosides. In this work, the method for efficient extraction and enrichment of gangliosides from brain was established. Moreover, the distribution of gangliosides in brain was obtained by matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). It was found that 3-aminoquinoline (3-AQ) as matrix was well-suited for MALDI MS analysis of gangliosides in negative ion mode. In addition, the pretreatment by ethanol (EtOH) cleaning brain section and the addition of ammonium formate greatly improved the MS signal of gangliosides in the brain section when MALDI MSI analysis was employed. The distribution of ganliosides in cerebral cortex, hippocampus and cerebellum was respectively acquired by electrospray ionization (ESI) MS and MALDI MSI, and the data were compared for reliability evaluation of MALDI MSI. Further, applying MALDI MSI technology, the distribution of gangliosides in amyloid precursor protein transgenic mouse brain was obtained, which may provide a new insight for bioresearch of Alzheimer's disease (AD).
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