ArticleBMC genomics2016
Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 98 citations in OpenAlex.
- Integrative Methylome and Transcriptome Analysis of DNA Methylation and Sex-Biased Gene Expression in Northern Pike (Animals : an open access journal from MDPI · 2026Article
- Integrated Analysis of mRNA-seq and miRNA-seq Reveal the Dynamics of the Gonadal Differentiation of Procypris Mera.Marine biotechnology (New York, N.Y.) · 2026Article
- Sexual Antagonism and Sex Determination in Three Syngnathid Species Alongside a Male Pregnancy Gradient.Genome biology and evolution · 2025Article
- Integrated Analysis of Differential Expression Profiles of miRNA and mRNA in Gonads ofAnimals : an open access journal from MDPI · 2025Article
- The potential regulatory role of non-coding RNAs in mifepristone-induced masculinization in Takifugu rubripes gonads.BMC genomics · 2025Article
- Identification of sex-biased MiRNA markers informative of heat-past events.BMC genomics · 2025Article
- A detailed transcriptome study uncovers the epigenetic characteristics associated with Aromatase inhibitor-induced masculinization in Takifugu rubripes larvae gonads.BMC genomics · 2025Article
- Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages.Marine biotechnology (New York, N.Y.) · 2025Article
- Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens.Frontiers in veterinary science · 2024Article
- microRNA-mRNA Analysis Reveals Tissue-Specific Regulation of microRNA in Mangrove Clam (Biology · 2023Article
- Deciphering sex-specific miRNAs as heat-recorders in zebrafish.Scientific reports · 2022Article
- Article
- Identification and Characterization of miRNAs and Their Predicted mRNAs in the Larval Development of Pearl Oyster Pinctada fucata.Marine biotechnology (New York, N.Y.) · 2022Article
- Comprehensive Characterization of Circular RNAs in Ovary and Testis From Nile Tilapia.Frontiers in veterinary science · 2022Article
- MicroRNAs May Play an Important Role in Sexual Reversal Process of Chinese Soft-Shelled Turtle,Genes · 2021Article
- Integrated Analysis of miR-430 on Steroidogenesis-Related Gene Expression of Larval Rice Field EelInternational journal of molecular sciences · 2021Article
- Integrated Analysis of mRNA- and miRNA-Seq in the Ovary of Rare MinnowFrontiers in genetics · 2021Article
- Identification and Characterization of Osmoregulation Related MicroRNAs in Gills of Hybrid Tilapia Under Three Types of Osmotic Stress.Frontiers in genetics · 2021Article
- GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish.Frontiers in genetics · 2021Article
- Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream (International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) represent a second regulatory network that has important effects on gene expression and protein translation during biological process. However, the possible role of miRNAs in the early stages of fish sex differentiation is not well understood. In this study, we carried an integrated analysis of miRNA and mRNA expression profiles to explore their possibly regulatory patterns at the critical stage of sex differentiation in tilapia.
resultsWe identified 279 pre-miRNA genes in tilapia genome, which were highly conserved in other fish species. Based on small RNA library sequencing, we identified 635 mature miRNAs in tilapia gonads, in which 62 and 49 miRNAs showed higher expression in XX and XY gonads, respectively. The predicted targets of these sex-biased miRNAs (e.g., miR-9, miR-21, miR-30a, miR-96, miR-200b, miR-212 and miR-7977) included genes encoding key enzymes in steroidogenic pathways (Cyp11a1, Hsd3b, Cyp19a1a, Hsd11b) and key molecules involved in vertebrate sex differentiation (Foxl2, Amh, Star1, Sf1, Dmrt1, and Gsdf). These genes also showed sex-biased expression in tilapia gonads at 5 dah. Some miRNAs (e.g., miR-96 and miR-737) targeted multiple genes involved in steroid synthesis, suggesting a complex miRNA regulatory network during early sex differentiation in this fish.
conclusionsThe sequence and expression patterns of most miRNAs in tilapia are conserved in fishes, indicating the basic functions of vertebrate miRNAs might share a common evolutionary origin. This comprehensive analysis of miRNA and mRNA at the early stage of molecular sex differentiation in tilapia XX and XY gonads lead to the discovery of differentially expressed miRNAs and their putative targets, which will facilitate studies of the regulatory network of molecular sex determination and differentiation in fishes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.