ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2016
Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats.
Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 40 citations in OpenAlex.
- Assessing the translational value of animal models in developing pain-targeting therapies for clinical osteoarthritis.Communications medicine · 2026Review
- Phytochemistry, Pharmacological Potential and Industrial Applications ofPharmaceuticals (Basel, Switzerland) · 2026Review
- Effects of single and combination dosage regimens of ketorolac and dexmedetomidine on allodynia and nociception in rat model of osteoarthritis: an animal multi-group trial.BMC anesthesiology · 2026Article
- Ricin Toxicity to Intestinal Cells Leads to Multiple Cell Death Pathways Mediated by Oxidative Stress.Toxins · 2025Article
- Therapeutic potential of Lawsonia inermis Linn: a comprehensive overview.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Hyaluronic acid/diminazene aceturate combination ameliorates osteoarthritic anomalies in a rodent model: a role of the ACE2/Ang1-7/MasR axis.Inflammopharmacology · 2023Article
- Diminazene aceturate or losartan ameliorates the functional, radiological and histopathological alterations in knee osteoarthritis rodent model: repurposing of the ACE2/Ang1-7/MasR cascade.Journal of experimental orthopaedics · 2023Article
- Effects ofIranian journal of pharmaceutical research : IJPR · 2022Article
- Hydroethanolic extract ofResearch in pharmaceutical sciences · 2022Article
- Review
- Proteoglycans isolated from the bramble shark cartilage show potential anti-osteoarthritic properties.Inflammopharmacology · 2019Article
- Plants Producing Ribosome-Inactivating Proteins in Traditional Medicine.Molecules (Basel, Switzerland) · 2016Review
- Protective effect of Henna (Avicenna journal of phytomedicineArticle
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMany plants have been introduced in Iranian traditional medicine for treatment of different joint problems including knee pain. Topical application of the mixture of Lawsonia inermis L. leaves (Henna) with aqueous extract of Ricinus communis L. leaves have been mentioned to have significant effects on reducing knee pain. The present study was designed to evaluate the analgesic and anti-inflammatory effects of the mixture of these two herbs in male rats.
methodsWe induced knee osteoarthritis as a model of chronic pain by intra-articular injection of mono sodium iodoacetate (MIA). Mechanical allodynia, hotplate latency test, spontaneous movements and gait analysis were used for the evaluation of analgesic activity. Anti-inflammatory activity was evaluated by measuring the diameter and the volume of the injected paw compared to contralateral paw. These tests were monitored at days 1, 3, 7, 14 and 21 of MIA administration. Histopathological evaluations were also used to assess the efficacy of the treatment on inflammation and lesions in knee tissue. In all tests, diclofenac topical gel was used as a positive control. The herbal extracts, their mixture, and vehicle or diclofenac gel were administered daily for 14 days by topical route.
resultsThe mixture of these two extracts significantly reduced the knee joint width and volume of the injected paws and also improved foot prints in gait analysis after 3 days of MIA injection. Analysis of mechanical allodynia (after 21 days), hotplate latency test (after 10 days), spontaneous movements (after 7 days) and in positive control group (after 3 days in all tests and in mechanical allodynia after 14 days) compared to the vehicle group, showed significant effects. Topical usage of the selected formulation made significant histopathological changes on the knee of the rats. Compared to the vehicle group, the tests and diclofenac groups showed less reactions characterized by negligible edema and a few scattered inflammatory lymphoid cells.
conclusionThe present findings showed that the present formulation not only was able to mitigate pain and inflammation in the paws but also made significant histopathological changes on the knee of the rats. Further studies are necessary to confirm the effect of the formulation.
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