ArticleBMC developmental biology2016
Live imaging of muscle histolysis in Drosophila metamorphosis.
Article in BMC developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Mating triggers a physiological switch to reproduction in field crickets to mitigate the flight-oogenesis tradeoff.Current research in insect science · 2026Article
- Dual-purpose dynamics emerge from a heterogeneous cell population in Drosophila metamorphosis.PLoS computational biology · 2025Article
- Muscle degeneration in aging Drosophila flies: the role of mechanical stress.Skeletal muscle · 2024Article
- Understanding Developmental Cell Death UsingCells · 2024Review
- Integration of proteomic and genetic approaches to assess developmental muscle atrophy.The Journal of experimental biology · 2021Article
- Development of the indirect flight muscles of Aedes aegypti, a main arbovirus vector.BMC developmental biology · 2021Article
- Tissue-specific Nrf2 signaling protects against methylmercury toxicity in Drosophila neuromuscular development.Archives of toxicology · 2020Article
- Review
- Comparative proteomics provides insights into diapause program of Bactrocera minax (Diptera: Tephritidae).PloS one · 2020Article
- Drosophila metamorphosis involves hemocyte mediated macroendocytosis and efferocytosis.The International journal of developmental biology · 2020Article
- Visualization of insect metamorphosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Thin is required for cell death in the Drosophila abdominal muscles by targeting DIAP1.Cell death & disease · 2018Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.
methodTo identify and characterize genes that control the decision between survival and cell death of muscles, we developed a method comprising in vivo imaging, targeted gene perturbation and time-lapse image analysis. Our approach enabled us to study the cytological and temporal aspects of abnormal cell death phenotypes.
resultsIn a previous genetic screen for genes controlling muscle size and cell death in metamorphosis, we identified gene perturbations that induced cell death of persistent or inhibit histolysis of doomed larval muscles. RNA interference (RNAi) of the genes encoding the helicase Rm62 and the lysosomal Cathepsin-L homolog Cysteine proteinase 1 (Cp1) caused premature cell death of persistent muscle in early and mid-pupation, respectively. Silencing of the transcriptional co-repressor Atrophin inhibited histolysis of doomed muscles. Overexpression of dominant-negative Target of Rapamycin (TOR) delayed the histolysis of a subset of doomed and induced ablation of all persistent muscles. RNAi of AMPKα, which encodes a subunit of the AMPK protein complex that senses AMP and promotes ATP formation, led to loss of attachment and a spherical morphology. None of the perturbations affected the survival of newly formed adult muscles, suggesting that the method is useful to find genes that are crucial for the survival of metabolically challenged muscles, like those undergoing atrophy. The ablation of persistent muscles did not affect eclosion of adult flies.
conclusionsLive imaging is a versatile approach to uncover gene functions that are required for the survival of muscle undergoing remodelling, yet are dispensable for other adult muscles. Our approach promises to identify molecular mechanisms that can explain the resilience of muscles to PCD.
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