ArticleEuropean journal of microbiology & immunology2016
Evidence of In Vivo Existence of Borrelia Biofilm in Borrelial Lymphocytomas.
Article in European journal of microbiology & immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 79 citations in OpenAlex.
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- Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes.Scientific reports · 2024Article
- Screening Marine Microbial Metabolites as Promising Inhibitors ofBioMed research international · 2024Article
- Members of the paralogous gene family 12 from the Lyme disease agent Borrelia burgdorferi are non-specific DNA-binding proteins.PloS one · 2024Article
- Article
- Global transcriptomic analysis of breast cancer and normal mammary epithelial cells infected with Borrelia burgdorferi.European journal of microbiology & immunology · 2023Article
- Borrelia burgdorferi-A Bacterium Worthy of Consideration in Culture-Negative Prosthetic Joint Infection.Journal of the American Academy of Orthopaedic Surgeons. Global research & reviews · 2023Article
- The Use of Natural Bioactive Nutraceuticals in the Management of Tick-Borne Illnesses.Microorganisms · 2023Review
- Inhibition ofInternational journal of molecular sciences · 2022Article
- Large-Scale Sequencing ofGenes · 2022Review
- Efficacy of Short-Term High Dose Pulsed Dapsone Combination Therapy in the Treatment of Chronic Lyme Disease/Post-Treatment Lyme Disease Syndrome (PTLDS) and Associated Co-Infections: A Report of Three Cases and Literature Review.Antibiotics (Basel, Switzerland) · 2022Article
- Persistent Lyme disease with cutaneous Borrelia biofilm formation.The British journal of dermatology · 2022Article
- Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis.Microorganisms · 2022Review
- Borrelia burgdorferi Co-Localizing with Amyloid Markers in Alzheimer's Disease Brain Tissues.Journal of Alzheimer's disease : JAD · 2022Article
- In Vivo Biofilm Formation of PathogenicMicroorganisms · 2021Article
- Leptospira interrogans biofilm formation in Rattus norvegicus (Norway rats) natural reservoirs.PLoS neglected tropical diseases · 2021Article
- The Brilliance ofPathogens (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lyme borreliosis, caused by the spirochete Borrelia burgdorferi sensu lato, has grown into a major public health problem. We recently identified a novel morphological form of B. burgdorferi, called biofilm, a structure that is well known to be highly resistant to antibiotics. However, there is no evidence of the existence of Borrelia biofilm in vivo; therefore, the main goal of this study was to determine the presence of Borrelia biofilm in infected human skin tissues. Archived skin biopsy tissues from borrelial lymphocytomas (BL) were reexamined for the presence of B. burgdorferi sensu lato using Borrelia-specific immunohistochemical staining (IHC), fluorescent in situ hybridization, combined fluorescent in situ hybridization (FISH)-IHC, polymerase chain reaction (PCR), and fluorescent and atomic force microscopy methods. Our morphological and histological analyses showed that significant amounts of Borrelia-positive spirochetes and aggregates exist in the BL tissues. Analyzing structures positive for Borrelia showed that aggregates, but not spirochetes, expressed biofilm markers such as protective layers of different mucopolysaccharides, especially alginate. Atomic force microscopy revealed additional hallmark biofilm features of the Borrelia/alginate-positive aggregates such as inside channels and surface protrusions. In summary, this is the first study that demonstrates the presence of Borrelia biofilm in human infected skin tissues.
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Registered trials
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