ArticleScientific reports2016
Posing for a picture: vesicle immobilization in agarose gel.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Bioinspired organic materials for seamless neurohybrid interfaces: from material design to living electronics.Materials horizons · 2026Review
- Flip Flop-Associated Growth and Division of Synthetic Cells.ACS nano · 2026Article
- Reconstitution of Active Plant HBio-protocol · 2026Article
- TMEM63 proteins act as mechanically activated cholesterol modulated lipid scramblases contributing to membrane mechano-resilience.Nature communications · 2026Article
- Model Lipid Bilayers as Platforms for Replicating Bacterial Membranes: A Study of Dynamics and Structural Heterogeneity.The journal of physical chemistry. B · 2026Article
- Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Liposome-EncapsulatedACS synthetic biology · 2025Article
- Bioprinting of Synthetic Cell-like Lipid Vesicles to Augment the Functionality of Tissues after Manufacturing.ACS synthetic biology · 2024Article
- Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers.Scientific reports · 2023Article
- Biomimetic asymmetric bacterial membranes incorporating lipopolysaccharides.Biophysical journal · 2023Article
- Efficient liposome fusion to phase-separated giant vesicles.Biophysical journal · 2023Article
- Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review.Marine drugs · 2023Review
- Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.International journal of molecular sciences · 2023Article
- A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets.Membranes · 2023Review
- A simple method to make, trap and deform a vesicle in a gel.Scientific reports · 2023Article
- Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins.iScience · 2023Article
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Molecular sensors reveal the mechano-chemical response ofCell surface (Amsterdam, Netherlands) · 2022Article
- GM1 asymmetry in the membrane stabilizes pores.Biophysical journal · 2022Article
- Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles.Soft matter · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Taking a photo typically requires the object of interest to stand still. In science, imaging is potentiated by optical and electron microscopy. However, living and soft matter are not still. Thus, biological preparations for microscopy usually include a fixation step. Similarly, immobilization strategies are required for or substantially facilitate imaging of cells or lipid vesicles, and even more so for acquiring high-quality data via fluorescence-based techniques. Here, we describe a simple yet efficient method to immobilize objects such as lipid vesicles with sizes between 0.1 and 100 μm using agarose gel. We show that while large and giant unilamellar vesicles (LUVs and GUVs) can be caged in the pockets of the gel meshwork, small molecules, proteins and micelles remain free to diffuse through the gel and interact with membranes as in agarose-free solutions, and complex biochemical reactions involving several proteins can proceed in the gel. At the same time, immobilization in agarose has no adverse effect on the GUV size and stability. By applying techniques such as FRAP and FCS, we show that the lateral diffusion of lipids is not affected by the gel. Finally, our immobilization strategy allows capturing high-resolution 3D images of GUVs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.