Evidence map›Paper›PMID 27140521›Full record

ArticleUltrasound in medicine & biology2016

Non-Invasive Ultrasound Liver Ablation Using Histotripsy: Chronic Study in an In Vivo Rodent Model.

Eli Vlaisavljevich, Joan Greve, Xu Cheng, Kimberly Ives, Jiaqi Shi, Lifang Jin, Alexa Arvidson, Tim Hall, Theodore H Welling, Gabe Owens and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ultrasound in medicine & biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.

  1. Histotripsy: the first noninvasive, non-ionizing, non-thermal ablation technique based on ultrasound.International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2021
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  15. Overview of Therapeutic Ultrasound Applications and Safety Considerations: 2024 Update.Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine · 2025
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  17. Histotripsy: A Method for Mechanical Tissue Ablation with Ultrasound.Annual review of biomedical engineering · 2024
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  20. Insights fromInternational journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Eli VlaisavljevichDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA. Electronic address: evlaisav@umich.edu.
Joan GreveDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Xu ChengDepartment of Urology, University of Michigan, Ann Arbor, MI, USA.
Kimberly IvesDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Jiaqi ShiDepartment of Pathology, University of Michigan, Ann Arbor, MI, USA.
Lifang JinDepartment of Ultrasound, Shanghai Jiaotong University, Shanghai, China.
Alexa ArvidsonDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Tim HallDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Theodore H WellingDepartment of Surgery, University of Michigan, Ann Arbor, MI, USA.
Gabe OwensDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA.
William RobertsDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Urology, University of Michigan, Ann Arbor, MI, USA.
Zhen XuDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA.
University of Michigan–Ann Arbor · USShanghai Jiao Tong University · CN

Funding

Image-Guided Non-Invasive Ultrasonic Thrombolysis Using HistotripsyR01EB008998 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI XU, ZHEN · 2008 to 2017
$4.5M
NIBIB NIH HHS R01 EB008998
6 · The paper itself

Abstract

Hepatocellular carcinoma, or liver cancer, has the fastest growing incidence among cancers in the United States. Current liver ablation methods are thermal-based and share limitations due to the heat sink effect from the blood flow through the highly vascular liver. Recently, our group has investigated histotripsy as a non-invasive liver cancer ablation method. Histotripsy is a non-thermal ultrasonic ablation method that fractionates tissue through the control of acoustic cavitation. Previous experiments in an in vivo porcine model show that histotripsy can create well-confined lesions in the liver through ribcage obstruction without damaging the overlying ribs and other tissues. Histotripsy can also completely fractionate liver tissue surrounding major vessels while preserving the vessels. In this study, we investigate the long-term effects of histotripsy liver ablation in a rodent model. We hypothesize that the fractionated histotripsy lesion will be resorbed by the liver, resulting in effective tissue healing. To test this hypothesis, the livers of 20 healthy rats were treated with histotripsy using an 8-element 1-MHz histotripsy transducer. Rats were euthanized after 0, 3, 7, 14 and 28 days (n = 4). In vivo and post mortem results showed histotripsy lesions were successfully generated through the intact abdomen in all 20 rats. Magnetic resonance imaging found primarily negative contrast on day 0, positive contrast on day 3 and rapid normalization of signal intensity thereafter (i.e., signal amplitude returned to baseline levels seen in healthy liver tissue). Histologically, lesions were completely fractionated into an acellular homogenate. The lesions had a maximum cross-sectional area of 17.2 ± 1.9 mm(2) and sharp boundaries between the lesion and the healthy surrounding tissue after treatment. As the animals recovered after treatment, the histotripsy tissue homogenate was almost completely replaced by regenerated liver parenchyma, resulting in a small fibrous lesion (<1 mm(2) maximum cross-section) remaining after 28 d. The results of this study suggest that histotripsy has potential as a non-invasive liver ablation method for effective tissue removal.

Indexed as

AnimalsHigh-Intensity Focused Ultrasound AblationLiverMaleModels, AnimalRatsRats, Sprague-DawleyCavitationHistotripsyLiver cancerNon-invasive tissue ablationUltrasound

Identifiers

PMID27140521
PMCPMC4912895
OpenAlexW2344956073

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.