ArticleVirus genes2016
The high-throughput production of dsRNA against sacbrood virus for use in the honey bee Apis cerana (Hymenoptera: Apidae).
Article in Virus genes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Article
- Conserved Genes in Highly Regenerative Metazoans Are Associated with Planarian Regeneration.Genome biology and evolution · 2024Article
- Effects of Deformed Wing Virus-Targeting dsRNA on Viral Loads in Bees Parasitised and Non-Parasitised byViruses · 2023Article
- Can egg yolk antibodies terminate the CSBV infection in apiculture?Virus research · 2023Review
- Large-Scale Application of Double-Stranded RNA Shows Potential for Reduction of Sacbrood Virus Disease inViruses · 2023Article
- Review
- Virome Analysis Reveals Diverse and Divergent RNA Viruses in Wild Insect Pollinators in Beijing, China.Viruses · 2022Article
- Antiviral Activities of a Medicinal Plant Extract Against Sacbrood Virus in Honeybees.Virology journal · 2021Article
- Advances in the Development of Microbial Double-Stranded RNA Production Systems for Application of RNA Interference in Agricultural Pest Control.Frontiers in bioengineering and biotechnology · 2021Review
- Effects of Tropilaelaps mercedesae on midgut bacterial diversity of Apis mellifera.Experimental & applied acarology · 2019Article
- Scientific Advances in ControllingFrontiers in veterinary science · 2019Review
- The Wisdom of Honeybee Defenses Against Environmental Stresses.Frontiers in microbiology · 2018Review
- Diverse Factors Affecting Efficiency of RNAi in Honey Bee Viruses.Frontiers in genetics · 2018Article
- RNA Interference in Insects: Protecting Beneficials and Controlling Pests.Frontiers in physiology · 2018Review
- Preparation and Application of Egg Yolk Antibodies Against Chinese Sacbrood Virus Infection.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sacbrood virus (SBV) is a serious threat to honey bees. Currently, there is no specific drug available for the treatment of SBV that does not affect the quality of the bee product. RNA interference (RNAi) is an important antiviral strategy for disease control. To effectively utilize this technology, the large-scale production and purification of double-stranded RNA (dsRNA) is necessary. Here, a dsRNA-expressing plasmid targeting the VP1 gene of Chinese sacbrood virus (CSBV) was constructed and expressed in Escherichia coli (E. coli) HT115 (DE3). After lysing and ethanol precipitation from E. coli, dsRNA VP1 was purified with an anion exchange chromatography column. Second instar larvae of Apis cerana were fed the purified dsRNA VP1. A significant decrease in larval mortality and the level of expression of the VP1 gene after CSBV infection was demonstrated after the ingestion of dsRNA VP1. This result provides a potential method for the large-scale production of dsRNA to protect A. cerana from CSBV infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.