Evidence map›Paper›PMID 27139626›Full record

ArticleActa crystallographica. Section D, Structural biology2016

A generic protocol for protein crystal dehydration using the HC1b humidity controller.

Carina M C Lobley, James Sandy, Juan Sanchez-Weatherby, Marco Mazzorana, Tobias Krojer, Radosław P Nowak, Thomas L Sorensen

Abstract read
In one paragraph

Article in Acta crystallographica. Section D, Structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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  8. Merging of synchrotron serial crystallographic data by a genetic algorithm.Acta crystallographica. Section D, Structural biology · 2016
    Article
  9. In vacuo X-ray data collection from graphene-wrapped protein crystals.Acta crystallographica. Section D, Biological crystallography · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Carina M C LobleyDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
James SandyDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
Juan Sanchez-WeatherbyDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
Marco MazzoranaDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
Tobias KrojerStructural Genomics Consortium, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7DQ, England.
Radosław P NowakStructural Genomics Consortium, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7DQ, England.
Thomas L SorensenDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.

Funding

CIHRWellcome TrustWellcome Trust 092809/Z/10/Z
6 · The paper itself

Abstract

Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and complexity. This study uses a high-precision crystal humidifier/dehumidifier to provide an airstream of known relative humidity in which the crystals are mounted: a precise yet hassle-free approach to altering crystal hydration. A protocol is introduced to assess the impact of crystal dehydration systematically applied to nine experimental crystal systems. In one case, that of glucose isomerase, dehydration triggering a change of space group from I222 to P21212 was observed. This observation is supported by an extended study of the behaviour of the glucose isomerase crystal structure during crystal dehydration.

Indexed as

Aldose-Ketose IsomerasesBacterial ProteinsCrystallizationCrystallography, X-RayDatabases, ProteinDesiccationEndopeptidase KEquipment DesignFungal ProteinsFungiHumansHumidityModels, MolecularProtein ConformationProteinsStreptomycesAldose-Ketose IsomerasesBacterial ProteinsEndopeptidase KFungal ProteinsProteinsxylose isomerasecryoprotectioncrystal dehydrationgeneric protocolhumidity controlmacromolecular crystallizationroom temperature

Identifiers

PMID27139626
PMCPMC4854313

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.