ArticleActa crystallographica. Section D, Structural biology2016
Transmission electron microscopy for the evaluation and optimization of crystal growth.
Article in Acta crystallographica. Section D, Structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Structural basis of transcription: RNA polymerase II substrate binding and metal coordination using a free-electron laser.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.IUCrJ · 2022Article
- MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.Nature communications · 2021Article
- Macromolecular crystallography using microcrystal electron diffraction.Acta crystallographica. Section D, Structural biology · 2021Review
- MicroED Sample Preparation and Data Collection For Protein Crystals.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Detection of Microcrystals for CryoEM.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Rapid screening ofJournal of applied crystallography · 2020Article
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
- The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The cryo-EM method microcrystal electron diffraction (MicroED).Nature methods · 2019Review
- Transcription with a laser: Radiation-damage-free diffraction of RNA Polymerase II crystals.Methods (San Diego, Calif.) · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- MicroED: a versatile cryoEM method for structure determination.Emerging topics in life sciences · 2018Article
- MicroED Structure of AuThe journal of physical chemistry letters · 2017Article
- Protein structure determination by electron diffraction using a single three-dimensional nanocrystal.Acta crystallographica. Section D, Structural biology · 2017Article
- Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.Nature methods · 2017Article
- Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography.Archives of biochemistry and biophysics · 2016Review
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22 authors.
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Abstract
The crystallization of protein samples remains the most significant challenge in structure determination by X-ray crystallography. Here, the effectiveness of transmission electron microscopy (TEM) analysis to aid in the crystallization of biological macromolecules is demonstrated. It was found that the presence of well ordered lattices with higher order Bragg spots, revealed by Fourier analysis of TEM images, is a good predictor of diffraction-quality crystals. Moreover, the use of TEM allowed (i) comparison of lattice quality among crystals from different conditions in crystallization screens; (ii) the detection of crystal pathologies that could contribute to poor X-ray diffraction, including crystal lattice defects, anisotropic diffraction and crystal contamination by heavy protein aggregates and nanocrystal nuclei; (iii) the qualitative estimation of crystal solvent content to explore the effect of lattice dehydration on diffraction and (iv) the selection of high-quality crystal fragments for microseeding experiments to generate reproducibly larger sized crystals. Applications to X-ray free-electron laser (XFEL) and micro-electron diffraction (microED) experiments are also discussed.
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