ArticlePloS one2016
Identification and Characterization of Two Novel RNA Viruses from Anopheles gambiae Species Complex Mosquitoes.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Pooled it
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- A Systematic Review on the Viruses ofTropical medicine and infectious disease · 2023Review
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- Novel Dicistroviruses in an Unexpected Wide Range of Invertebrates.Food and environmental virology · 2021Article
- Sensitivity and specificity of metatranscriptomics as an arbovirus surveillance tool.Scientific reports · 2019Article
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- De novo profiling of RNA viruses in Anopheles malaria vector mosquitoes from forest ecological zones in Senegal and Cambodia.BMC genomics · 2019Article
- Small RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection.Insect biochemistry and molecular biology · 2019Article
- Exploiting the Legacy of the Arbovirus Hunters.Viruses · 2019Review
- Interaction of RNA viruses of the natural virome with the African malaria vector, Anopheles coluzzii.Scientific reports · 2019Article
- Article
- Review
- Highly focused transcriptional response of Anopheles coluzzii to O'nyong nyong arbovirus during the primary midgut infection.BMC genomics · 2018Article
- Genetic characterization of a novel picorna-like virus in Culex spp. mosquitoes from Mozambique.Virology journal · 2018Article
- Metagenomic Analysis ofFrontiers in cellular and infection microbiology · 2018Article
- Metagenomic Sequencing From Mosquitoes in China Reveals a Variety of Insect and Human Viruses.Frontiers in cellular and infection microbiology · 2018Article
- Article
- Genomes of viral isolates derived from different mosquitos species.Virus research · 2017Article
Corrections and comments
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Authors and funding
14 authors at 8 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mosquitoes of the Anopheles gambiae complex display strong preference for human bloodmeals and are major malaria vectors in Africa. However, their interaction with viruses or role in arbovirus transmission during epidemics has been little examined, with the exception of O'nyong-nyong virus, closely related to Chikungunya virus. Deep-sequencing has revealed different RNA viruses in natural insect viromes, but none have been previously described in the Anopheles gambiae species complex. Here, we describe two novel insect RNA viruses, a Dicistrovirus and a Cypovirus, found in laboratory colonies of An. gambiae taxa using small-RNA deep sequencing. Sequence analysis was done with Metavisitor, an open-source bioinformatic pipeline for virus discovery and de novo genome assembly. Wild-collected Anopheles from Senegal and Cambodia were positive for the Dicistrovirus and Cypovirus, displaying high sequence identity to the laboratory-derived virus. Thus, the Dicistrovirus (Anopheles C virus, AnCV) and Cypovirus (Anopheles Cypovirus, AnCPV) are components of the natural virome of at least some anopheline species. Their possible influence on mosquito immunity or transmission of other pathogens is unknown. These natural viruses could be developed as models for the study of Anopheles-RNA virus interactions in low security laboratory settings, in an analogous manner to the use of rodent malaria parasites for studies of mosquito anti-parasite immunity.
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