ArticleStem cell reviews and reports2016
Efficient and Controlled Generation of 2D and 3D Bile Duct Tissue from Human Pluripotent Stem Cell-Derived Spheroids.
Article in Stem cell reviews and reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- A Systematic Review on Artificial Liver for Implantation.Journal of functional biomaterials · 2026Review
- Recent research progresses of bioengineered biliary stents.Materials today. Bio · 2024Review
- Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel.Nature communications · 2024Article
- Three-dimensional human bile duct formation from chemically induced human liver progenitor cells.Frontiers in bioengineering and biotechnology · 2023Article
- Directing Cholangiocyte Morphogenesis in Natural Biomaterial Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Advanced Nanotheranostics of CRISPR/Cas for Viral Hepatitis and Hepatocellular Carcinoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Organoids and Spheroids as Models for Studying Cholestatic Liver Injury and Cholangiocarcinoma.Hepatology (Baltimore, Md.) · 2021Review
- Intestinal organoids: roadmap to the clinic.American journal of physiology. Gastrointestinal and liver physiology · 2021Review
- Convergence of human pluripotent stem cell, organoid, and genome editing technologies.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Liver organoids in domestic animals: an expected promise for metabolic studies.Veterinary research · 2021Review
- Production of Functional Hepatobiliary Organoids from Human Pluripotent Stem Cells.International journal of stem cells · 2021Article
- 3D Printed Model of Extrahepatic Biliary Ducts for Biliary Stent Testing.Materials (Basel, Switzerland) · 2020Article
- Review
- Human-relevant preclinical in vitro models for studying hepatobiliary development and liver diseases using induced pluripotent stem cells.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Transient c-Src Suppression During Endodermal Commitment of Human Induced Pluripotent Stem Cells Results in Abnormal Profibrotic Cholangiocyte-Like Cells.Stem cells (Dayton, Ohio) · 2019Article
- Biliary Atresia Relevant Human Induced Pluripotent Stem Cells Recapitulate Key Disease Features in a Dish.Journal of pediatric gastroenterology and nutrition · 2019Article
- Development and characterization of cholangioids from normal and diseased human cholangiocytes as an in vitro model to study primary sclerosing cholangitis.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Review
- A process engineering approach to increase organoid yield.Development (Cambridge, England) · 2017Article
- Expression kinetics of hepatic progenitor markers in cellular models of human liver development recapitulating hepatocyte and biliary cell fate commitment.Experimental biology and medicine (Maywood, N.J.) · 2016Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
While in vitro liver tissue engineering has been increasingly studied during the last several years, presently engineered liver tissues lack the bile duct system. The lack of bile drainage not only hinders essential digestive functions of the liver, but also leads to accumulation of bile that is toxic to hepatocytes and known to cause liver cirrhosis. Clearly, generation of bile duct tissue is essential for engineering functional and healthy liver. Differentiation of human induced pluripotent stem cells (iPSCs) to bile duct tissue requires long and/or complex culture conditions, and has been inefficient so far. Towards generating a fully functional liver containing biliary system, we have developed defined and controlled conditions for efficient 2D and 3D bile duct epithelial tissue generation. A marker for multipotent liver progenitor in both adult human liver and ductal plate in human fetal liver, EpCAM, is highly expressed in hepatic spheroids generated from human iPSCs. The EpCAM high hepatic spheroids can, not only efficiently generate a monolayer of biliary epithelial cells (cholangiocytes), in a 2D differentiation condition, but also form functional ductal structures in a 3D condition. Importantly, this EpCAM high spheroid based biliary tissue generation is significantly faster than other existing methods and does not require cell sorting. In addition, we show that a knock-in CK7 reporter human iPSC line generated by CRISPR/Cas9 genome editing technology greatly facilitates the analysis of biliary differentiation. This new ductal differentiation method will provide a more efficient method of obtaining bile duct cells and tissues, which may facilitate engineering of complete and functional liver tissue in the future.
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