ArticlePloS one2016
The Interleukin-17 Induced Activation and Increased Survival of Equine Neutrophils Is Insensitive to Glucocorticoids.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- The IL-17A/Neutrophil axis plays a critical role in lethal infection induced by an emerging ultra-virulentVirulence · 2026Article
- Transcriptomic signatures reveal systemic adaptations and immune modulation in response to training and competitive racing in horses.Equine veterinary journal · 2026Article
- Selected cytokine and chemokine concentrations in equine autologous conditioned serum are similar under defined and practically relevant storage conditions.Frontiers in veterinary science · 2025Article
- Real-world effectiveness and safety of bimekizumab in Japanese patients with psoriasis: A single-center retrospective study.The Journal of dermatology · 2024Article
- Evaluating the effects of vitamin D Level on airway obstruction in two asthma endotypes in humans and in two mouse models with different intake of vitamin D during early-life.Frontiers in immunology · 2023Article
- The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.Animals : an open access journal from MDPI · 2022Review
- Effect of intrabronchial administration of autologous adipose-derived mesenchymal stem cells on severe equine asthma.Stem cell research & therapy · 2022Article
- Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences.Frontiers in immunology · 2022Article
- Article
- Glucocorticoid treatment in horses with asthma: A narrative review.Journal of veterinary internal medicine · 2021Review
- Review
- The role of neutrophils in asthma.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2021Review
- Neutrophils in Bronchoalveolar Lavage Fluid Indicating the Severity and Relapse of Pulmonary Sarcoidosis.Frontiers in medicine · 2021Article
- Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Equine Asthma: Current Understanding and Future Directions.Frontiers in veterinary science · 2020Review
- Chronic social stress Ameliorates psoriasiform dermatitis through upregulation of the Hypothalamic-Pituitary-Adrenal axis.Brain, behavior, and immunity · 2018Article
- Neutrophil extracellular traps are downregulated by glucocorticosteroids in lungs in an equine model of asthma.Respiratory research · 2017Article
- Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses.Scientific reports · 2017Article
- 1,25-dihydroxyvitamin D3 reduces mouse airway inflammation of neutrophilic asthma by transcriptional modulation of interleukin-17A.American journal of translational research · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlucocorticoids (GCs) are the most effective drugs for the treatment of human asthma. However, a subgroup of asthmatic patients with neutrophilic airway inflammation is insensitive to GCs. Interleukin-17 (IL-17), a cytokine upregulated in the airways of a subset of human asthmatic patients, contributes to the recruitment of neutrophils and induces a glucocorticoid resistance in human airway epithelial cells. We hypothesized that IL-17 similarly activates neutrophils and contributes to their persistence in the asthmatic airways in spite of glucocorticoid therapy.
objectiveTo determine whether IL-17 directly activates neutrophils and whether this response is attenuated by GCs.
methodsNeutrophils were isolated from the blood of horses and incubated in the presence of recombinant equine IL-17, LPS and dexamethasone. mRNA and protein expression of IL-17 receptors (IL-17RA/IL-17RC) were assessed by qPCR and immunoblot, respectively. Pro-inflammatory cytokine expression, cell viability and apoptosis were determined by qPCR, Trypan Blue exclusion test, and flow cytometry, respectively.
resultsEquine neutrophils express both IL-17RA and IL-17RC at the mRNA and protein levels. Neutrophil stimulation with IL-17 increases the mRNA expression of IL-8, which is not attenuated by dexamethasone (p = 0.409). Also, neutrophil viability is significantly increased (p<0.0001) by IL-17 in the presence of LPS when compared to LPS alone. Flow cytometry and light microscopy revealed that LPS-induced apoptosis is decreased by IL-17 (p = 0.02 and p = 0.006 respectively).
conclusionThese results indicate that IL-17 directly activates equine neutrophils at 24 hours, and that the expression of IL-8 thus induced is not attenuated by GCs. Additionally, IL-17 increases neutrophil viability and decreases apoptosis. These findings suggest an important role of IL-17 in pulmonary persistence of neutrophils in the asthmatic airways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.