ArticlePlants (Basel, Switzerland)2013
Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid.
Article in Plants (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor.Scientific reports · 2022Article
- A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus.Scientific reports · 2022Article
- Article
- Two-step method for isolation of high-quality RNA from stored seeds of maize rich in starch.3 Biotech · 2020Article
- RNA-seq data from whole rice grains of pigmented and non-pigmented Malaysian rice varieties.Data in brief · 2020Article
- A Combined RNA Preservation and Extraction Protocol for Gene Expression Studies in Cacao Beans.Frontiers in plant science · 2020Article
- Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseedPlant methods · 2020Article
- Article
- 5-Azacitidine Induces Cell Death in a Tissue Culture ofInternational journal of molecular sciences · 2018Article
- Decline in RNA integrity of dry-stored soybean seeds correlates with loss of germination potential.Journal of experimental botany · 2017Article
- A High-Throughput RNA Extraction for Sprouted Single-Seed Barley (Hordeum vulgare L.) Rich in Polysaccharides.Plants (Basel, Switzerland) · 2016Article
- A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties.BMC plant biology · 2016Article
- Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm.Protoplasma · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Jerusalem artichoke (Helianthus tuberosus L.) is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis challenging. This study was aimed to improve existing methods for extracting total RNA from Jerusalem artichoke dry seeds and to assess the applicability of the improved method in other plant species. Five RNA extraction methods were evaluated on Jerusalem artichoke seeds and two were modified. One modified method with the significant improvement was applied to assay seeds of diverse Jerusalem artichoke accessions, sunflower, rice, maize, peanut and marigold. The effectiveness of the improved method to extract total RNA from seeds was assessed using qPCR analysis of four selected genes. The improved method of Ma and Yang (2011) yielded a maximum RNA solubility and removed most interfering substances. The improved protocol generated 29 to 41 µg RNA/30 mg fresh weight. An A260/A280 ratio of 1.79 to 2.22 showed their RNA purity. Extracted RNA was effective for downstream applications such as first-stranded cDNA synthesis, cDNA cloning and qPCR. The improved method was also effective to extract total RNA from seeds of sunflower, rice, maize and peanut that are rich in polyphenols, lipids and polysaccharides.
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