ArticleScientific reports2016
Inhibiting DNA methylation switches adipogenesis to osteoblastogenesis by activating Wnt10a.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 59 citations in OpenAlex.
- Systems genetics reveals ITIH5 as a key mediator of adipocyte-Endothelial crosstalk.Molecular metabolism · 2026Article
- DNA methylation and immune regulation in osteoporosis: emerging epigenetic targets for drug discovery.Frontiers in pharmacology · 2025Review
- Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells.Adipocyte · 2024Article
- Exploring epigenetic strategies for the treatment of osteoporosis.Molecular biology reports · 2024Review
- Effect of DNA methylation on the osteogenic differentiation of mesenchymal stem cells: concise review.Frontiers in genetics · 2024Review
- DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation.Biological research · 2023Article
- 5-Aza-2'-Deoxycytidine Regulates White Adipocyte Browning by Modulating miRNA-133a/Prdm16.Metabolites · 2022Article
- Targeted erasure of DNA methylation by TET3 drives adipogenic reprogramming and differentiation.Nature metabolism · 2022Article
- Constitutive loss of DNMT3A causes morbid obesity through misregulation of adipogenesis.eLife · 2022Article
- A Mixture of Endocrine Disrupting Chemicals Associated with Lower Birth Weight in Children Induces Adipogenesis and DNA Methylation Changes in Human Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
- Review
- AdipocyteCells · 2022Article
- Significant transcriptomic changes are associated with the inhibitory effects of 5-aza-2-deoxycytidine during adipogenic differentiation of MG-63 cells.Saudi journal of biological sciences · 2021Article
- Brown Fat Dnmt3b Deficiency Ameliorates Obesity in Female Mice.Life (Basel, Switzerland) · 2021Article
- Epigenetic interaction between UTX and DNMT1 regulates diet-induced myogenic remodeling in brown fat.Nature communications · 2021Article
- Emerging roles of epigenetic regulation in obesity and metabolic disease.The Journal of biological chemistry · 2021Review
- Article
- Regulation and Role of Transcription Factors in Osteogenesis.International journal of molecular sciences · 2021Review
- DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review.International journal of molecular sciences · 2021Review
- WNT Ligand Dependencies in Pancreatic Cancer.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Both adipocytes and osteoblasts share the mesodermal lineage that derives from mesenchymal stem cells. Most studies investigating the mechanisms underlying the regulation of adipogenic or osteoblastogenic development focus on transcriptional pathways; little is known about the epigenetic mechanisms in this process. We thus determined the role of 5-aza-2'-deoxycytidine (5-Aza-dC), an inhibitor of DNA methylation, in the lineage determination between adipogenesis and osteoblastogenesis. Inhibiting DNA methylation in 3T3-L1 preadipocytes by 5-Aza-dC significantly inhibited adipogenesis whereas promoted osteoblastogenesis. This dual effect of 5-Aza-dC was associated with up-regulation of Wnt10a, a key factor determining the fate of the mesenchymal lineage towards osteoblasts. Consistently, IWP-2, an inhibitor of Wnt proteins, was found to prevent the anti-adipogenic effect of 5-Aza-dC in 3T3-L1 preadipocytes and block the osteoblastogenic effect of 5-Aza-dC in ST2 mesenchymal stem cell line. Finally, the Wnt10a 5'-region is enriched with CpG sites, whose methylation levels were markedly reduced by 5-Aza-dC. Thus we conclude that inhibiting DNA methylation by 5-Aza-dC mutual-exclusively regulates the lineage determination of adipogenesis and osteoblastogenesis by demethylating Wnt10a gene and upregulating its expression. Our study defines DNA methylation as a novel mechanism underlying adipocyte and bone cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.