ArticleJournal of proteome research2016
Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging.
Article in Journal of proteome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024Pooled it
- Article
- Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Nature reviews. Methods primers · 2026Article
- Dissecting the role ofFrontiers in microbiology · 2026Article
- Understanding the development of bacterial colony: Physiology, new technology, and modeling.Quantitative biology (Beijing, China) · 2025Review
- Investigating the influence of diverse treatments on the autofluorescence properties of Bacillus subtilis.Scientific reports · 2025Article
- High-resolution 3D spatial distribution of complex microbial colonies revealed by mass spectrometry imaging.Journal of advanced research · 2025Article
- Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges.Microbiology and molecular biology reviews : MMBR · 2025Review
- Subtilosin A production is influenced by surfactin levels inmicroLife · 2025Article
- Matrix-assisted laser desorption/ionization mass spectrometry imaging for quorum sensing.AMB Express · 2024Review
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.Nature protocols · 2023Review
- Review
- Imaging mass spectrometry reveals complex lipid distributions acrossJournal of mass spectrometry and advances in the clinical lab · 2022Article
- Probing the growth and mechanical properties ofSynthetic and systems biotechnology · 2022Article
- "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.International journal of molecular sciences · 2022Review
- Surface-assisted laser desorption/ionization mass spectrometry imaging: A review.Mass spectrometry reviews · 2022Review
- Identification, Structure and Characterization ofJournal of clinical medicine · 2022Article
- Utilizing imaging mass spectrometry to analyze microbial biofilm chemical responses to exogenous compounds.Methods in enzymology · 2022Article
- Isocitrate dehydrogenase of Bacillus cereus is involved in biofilm formation.World journal of microbiology & biotechnology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Colony biofilms of Bacillus subtilis are a widely used model for studying cellular differentiation. Here, we applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to examine cellular and molecular heterogeneity in B. subtilis colony biofilms. From B. subtilis cells cultivated on a biofilm-promoting medium, we detected two cannibalistic factors not found in previous MALDI MSI studies of the same strain under different culturing conditions. Given the importance of cannibalism in matrix formation of B. subtilis biofilms, we employed a transcriptional reporter to monitor matrix-producing cell subpopulations using fluorescence imaging. These two complementary imaging approaches were used to characterize three B. subtilis strains, the wild type isolate NCIB3610, and two mutants, Δspo0A and ΔabrB, with defective and enhanced biofilm phenotypes, respectively. Upon deletion of key transcriptional factors, correlated changes were observed in biofilm morphology, signaling, cannibalistic factor distribution, and matrix-related gene expression, providing new insights on cannibalism in biofilm development. This work underscores the advantages of using multimodal imaging to compare spatial patterns of selected molecules with the associated protein expression patterns, obtaining information on cellular heterogeneity and function not obtainable when using a single method to characterize biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.