ArticleCell systems2016
Bistability in the Rac1, PAK, and RhoA Signaling Network Drives Actin Cytoskeleton Dynamics and Cell Motility Switches.
Article in Cell systems, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
115 citing papers in PubMed, 185 citations in OpenAlex.
- Long-range mutual activation establishes Rho and Rac polarity during cell migration.Nature cell biology · 2026Article
- A pore-forming toxin initiates ABI1 complex switching to promote bacterial cell-to-cell spread.Nature communications · 2026Article
- RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization.Journal of leukocyte biology · 2026Article
- PathCLAST: pathway-augmented contrastive learning with attention for interpretable spatial transcriptomics.Briefings in bioinformatics · 2026Article
- The effect and mechanism of total alkaloids ofFrontiers in pharmacology · 2026Article
- Mechanical observables of cancer invasion: actin, adhesion, and metastatic plasticity.Frontiers in cell and developmental biology · 2026Review
- Migrastatics: an overview of the current scenario and future cancer treatments.Frontiers in oncology · 2026Review
- PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration.bioRxiv : the preprint server for biology · 2025Article
- Rac1 in gastric cancer: a molecular driver of invasion, EMT, and therapeutic resistance.Journal of translational medicine · 2025Review
- AggreBots: Configuring CiliaBots through guided, modular tissue aggregation.Science advances · 2025Article
- Somatic mutations in IRE1α regulate keratinocyte migration and survival by differentially activating Rho GTPases.Journal of cell science · 2025Article
- The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion.BMC biology · 2025Article
- Effects of NaCell biochemistry and biophysics · 2025Review
- YAP controls cell migration and invasion through a Rho GTPase switch.Science signaling · 2025Article
- RABEP1 amplifies front signaling in neutrophil migration.bioRxiv : the preprint server for biology · 2025Article
- Dynamic clamping induces rotation-to-beating transition of pinned filaments in gliding assays.Journal of the Royal Society, Interface · 2025Article
- Colorectal cancer stem cells develop NK cell resistance via homotypic cell-in-cell structures suppressed by Stathmin1.Theranostics · 2025Article
- Model supports asymmetric regulation across the intercellular junction for collective cell polarization.PLoS computational biology · 2024Article
- RhoGDI in RBL-2H3 cells acts as a negative regulator of Rho GTPase signaling to inhibit granule exocytosis.Journal of leukocyte biology · 2024Article
- Blebology: principles of bleb-based migration.Trends in cell biology · 2024Review
55 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors at 6 institutions in 5 countries.
Funding
Abstract
Dynamic interactions between RhoA and Rac1, members of the Rho small GTPase family, play a vital role in the control of cell migration. Using predictive mathematical modeling, mass spectrometry-based quantitation of network components, and experimental validation in MDA-MB-231 mesenchymal breast cancer cells, we show that a network containing Rac1, RhoA, and PAK family kinases can produce bistable, switch-like responses to a graded PAK inhibition. Using a small chemical inhibitor of PAK, we demonstrate that cellular RhoA and Rac1 activation levels respond in a history-dependent, bistable manner to PAK inhibition. Consequently, we show that downstream signaling, actin dynamics, and cell migration also behave in a bistable fashion, displaying switches and hysteresis in response to PAK inhibition. Our results demonstrate that PAK is a critical component in the Rac1-RhoA inhibitory crosstalk that governs bistable GTPase activity, cell morphology, and cell migration switches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.