ArticleInternational journal of molecular sciences2016
Production of Bioactive Recombinant Bovine Chymosin in Tobacco Plants.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 25 citations in OpenAlex.
- Recombinant bovine chymosin expression in microalgae Chlamydomonas reinhardtii chloroplast: A step towards algal biomanufacturing of dairy enzymes.World journal of microbiology & biotechnology · 2026Article
- Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localisation.Plant biotechnology journal · 2026Article
- Tobacco Plant: A Novel and Promising Heterologous Bioreactor for the Production of Recombinant Bovine Chymosin.Molecular biotechnology · 2024Article
- Comprehensive bioinformatics-based annotation and functional characterization of bovine chymosin protein revealed novel biological insights.Food chemistry. Molecular sciences · 2024Article
- Biochemical Properties of a Promising Milk-Clotting Enzyme, Moose (Foods (Basel, Switzerland) · 2023Article
- Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts.BMC biotechnology · 2023Article
- Plant Aspartic Proteases for Industrial Applications: Thistle Get Better.Plants (Basel, Switzerland) · 2020Review
- Isolation and characterization of a novel seed-specific promoter from peanut (Arachis hypogaea L.).Molecular biology reports · 2019Article
- Article
- Association Analysis and Identification of ZmHKT1;5 Variation With Salt-Stress Tolerance.Frontiers in plant science · 2018Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chymosin (also known as rennin) plays an essential role in the coagulation of milk in the cheese industry. Chymosin is traditionally extracted from the rumen of calves and is of high cost. Here, we present an alternative method to producing bovine chymosin from transgenic tobacco plants. The CYM gene, which encodes a preprochymosin from bovine, was introduced into the tobacco nuclear genome under control of the viral 35S cauliflower mosaic promoter. The integration and transcription of the foreign gene were confirmed with Southern blotting and reverse transcription PCR (RT-PCR) analyses, respectively. Immunoblotting analyses were performed to demonstrate expression of chymosin, and the expression level was quantified by enzyme-linked immunosorbent assay (ELISA). The results indicated recombinant bovine chymosin was successfully expressed at an average level of 83.5 ng/g fresh weight, which is 0.52% of the total soluble protein. The tobacco-derived chymosin exhibited similar native milk coagulation bioactivity as the commercial product extracted from bovine rumen.
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