ArticleInvestigative ophthalmology & visual science2016
Inflammatory Response to Lipopolysaccharide on the Ocular Surface in a Murine Dry Eye Model.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- What is the impact of microbiota on dry eye: a literature review of the gut-eye axis.BMC ophthalmology · 2024Pooled it
- Translating Biomarker Discovery: From Bench to Bedside in Dry Eye Disease.International journal of molecular sciences · 2025Review
- Alterations of ocular surface microbiome in glaucoma and its association with dry eye.Journal of medical microbiology · 2025Article
- Ocular Surface Microbiota and Corneal Transplant Outcome: Is There a Link?Biomedicines · 2025Article
- Anti-inflammatory effects of LCB 03-0110 on human corneal epithelial and murine T helper 17 cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2025Article
- Review
- Effect of Topical Programmed Death-Ligand1 on Corneal Epithelium in Dry Eye Mouse.Biomolecules · 2024Article
- Protective effects of insulin on dry eye syndrome via TLR4/NF-κB pathway: based on network pharmacology andFrontiers in pharmacology · 2024Article
- Innate Immune System Activation, Inflammation and Corneal Wound Healing.International journal of molecular sciences · 2022Review
- Systems pharmacology dissection of the mechanisms and therapeutic potential of Cassiae semen for hepatoprotection and brightening eyes.Journal of food and drug analysis · 2022Article
- Sodium hyaluronate combined with rhEGF contributes to alleviate clinical symptoms and Inflammation in patients with Xerophthalmia after cataract surgery.BMC ophthalmology · 2022Article
- Oxytocin and secretin receptors - implications for dry eye syndrome and ocular pain.Frontiers in ophthalmology · 2022Review
- Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-Oxidative medicine and cellular longevity · 2022Article
- Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages.International journal of molecular sciences · 2021Article
- Conjunctival Commensal Isolation and Identification in Mice.Journal of visualized experiments : JoVE · 2021Article
- The ocular surface immune system through the eyes of aging.The ocular surface · 2021Review
- The Role of Endogenous Antimicrobial Peptides in Modulating Innate Immunity of the Ocular Surface in Dry Eye Diseases.International journal of molecular sciences · 2021Review
- Current Advances in Mechanisms and Treatment of Dry Eye Disease: Toward Anti-inflammatory and Immunomodulatory Therapy and Traditional Chinese Medicine.Frontiers in medicine · 2021Review
- The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface.PloS one · 2021Article
- Dysbiosis Modulates Ocular Surface Inflammatory Response to Liposaccharide.Investigative ophthalmology & visual science · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeToll-like receptor 4 (TLR4) alerts cells to the presence of bacteria by initiating an inflammatory response. We hypothesize that disruption of the ocular surface barrier in dry eye enhances TLR4 signaling. This study determined whether dry eye enhances expression of inflammatory mediators in response to topically applied TLR4 ligand.
methodsA single dose of lipopolysaccharide (LPS) or vehicle (endotoxin-free water) was applied to the cornea of nonstressed (NS) mice or mice subjected to 5 days of desiccating stress (DS). After 4 hours, corneal epithelium and conjunctiva were extracted to analyze expression of inflammatory mediators via PCR. Protein expression was confirmed by immunobead assay and immunostaining.
resultsTopically applied LPS increased expression of inflammatory mediators IL-1β, CXCL10, IL-12a, and IFN-γ in the conjunctiva, and IL-1β and CXCL10 in the cornea of NS mice compared to that in untreated controls. LPS in DS mice produced 3-fold increased expression of IL-1β in cornea and 2-fold increased expression in IL-12a in conjunctiva compared to that in LPS-treated control mice.
conclusionsLPS increased expression of inflammatory cytokines on the ocular surface. This expression was further increased in dry eye, which suggests that epithelial barrier disruption enhances exposure of LPS to TLR4+ cells and that the inflammatory response to endotoxin-producing commensal or pathogenic bacteria may be more severe in dry eye disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.