ArticleBlood transfusion = Trasfusione del sangue2016
Viral metagenomics applied to blood donors and recipients at high risk for blood-borne infections.
Article in Blood transfusion = Trasfusione del sangue, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Full annotation of serum virome in Chinese blood donors with elevated alanine aminotransferase levels.Transfusion · 2019Trial
- Review
- Human Anelloviruses: Influence of Demographic Factors, Recombination, and Worldwide Diversity.Microbiology spectrum · 2023Article
- Blood virosphere in febrile Tanzanian children.Emerging microbes & infections · 2021Article
- Deep Impact of Random Amplification and Library Construction Methods on Viral Metagenomics Results.Viruses · 2021Article
- Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia.Revista do Instituto de Medicina Tropical de Sao Paulo · 2021Article
- Beyond Cytomegalovirus and Epstein-Barr Virus: a Review of Viruses Composing the Blood Virome of Solid Organ Transplant and Hematopoietic Stem Cell Transplant Recipients.Clinical microbiology reviews · 2020Review
- Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus.PloS one · 2020Article
- Diversity of Vaginal Microbiome in Pregnancy: Deciphering the Obscurity.Frontiers in public health · 2020Review
- Broad Virus Detection and Variant Discovery in Fecal Samples of Hematopoietic Transplant Recipients Using Targeted Sequence Capture Metagenomics.Frontiers in microbiology · 2020Article
- Viral Metagenomics of Blood Donors and Blood-Derived Products Using Next-Generation Sequencing.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Review
- The Virome of Cerebrospinal Fluid: Viruses Where We Once Thought There Were None.Frontiers in microbiology · 2019Article
- Factors affecting the quality, safety and marketing approval of clotting factor concentrates for haemophilia.Blood transfusion = Trasfusione del sangue · 2018Review
- Shared and Distinct Features of Human Milk and Infant Stool Viromes.Frontiers in microbiology · 2018Article
- Article
- Review article: the human intestinal virome in health and disease.Alimentary pharmacology & therapeutics · 2017Review
- Integrating nested PCR with high-throughput sequencing to characterize mutations of HBV genome in low viral load samples.Medicine · 2017Article
- Efficient and unbiased metagenomic recovery of RNA virus genomes from human plasma samples.Scientific reports · 2017Article
Corrections and comments
- Comment on
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCharacterisation of human-associated viral communities is essential for epidemiological surveillance and to be able to anticipate new potential threats for blood transfusion safety. In high-resource countries, the risk of blood-borne agent transmission of well-known viruses (HBV, HCV, HIV and HTLV) is currently considered to be under control. However, other unknown or unsuspected viruses may be transmitted to recipients by blood-derived products. To investigate this, the virome of plasma from individuals at high risk for parenterally and sexually transmitted infections was analysed by high throughput sequencing (HTS). MATERIALS AND
methodsPurified nucleic acids from two pools of 50 samples from recipients of multiple transfusions, and three pools containing seven plasma samples from either HBV-, HCV- or HIV-infected blood donors, were submitted to HTS.
resultsSequences from resident anelloviruses and HPgV were evidenced in all pools. HBV and HCV sequences were detected in pools containing 3.8×10(3) IU/mL of HBV-DNA and 1.7×10(5) IU/mL of HCV-RNA, respectively, whereas no HIV sequence was found in a pool of 150 copies/mL of HIV-RNA. This suggests a lack of sensitivity in HTS performance in detecting low levels of virus. In addition, this study identified other issues, including laboratory contaminants and the uncertainty of taxonomic assignment of short sequence. No sequence suggestive of a new viral species was identified. DISCUSSION: This study did not identify any new blood-borne virus in high-risk individuals. However, rare and/or viruses present at very low titre could have escaped our protocol. Our results demonstrate the positive contribution of HTS in the detection of viral sequences in blood donations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.