ArticlePloS one2016
Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
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- An alternative model for maternal mtDNA inheritance.Nature genetics · 2025Article
- Off-target effects of mitochondrial oxidative phosphorylation inhibitors are common and can compromise the viability of cultured cells.Mitochondrial communications · 2025Article
- Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard.Developmental cell · 2024Article
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- Complex regulation of mitochondrial signaling by human adenovirus minor capsid protein VI.Journal of virology · 2024Article
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- Lactate activates the mitochondrial electron transport chain independently of its metabolism.Molecular cell · 2023Article
- Generation of Mammalian Cells Devoid of Mitochondrial DNA (ρCurrent protocols · 2023Article
- Mesoscale structure-function relationships in mitochondrial transcriptional condensates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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- A genome on shaky ground: exploring the impact of mitochondrial DNA integrity on Parkinson's disease by highlighting the use of cybrid models.Cellular and molecular life sciences : CMLS · 2022Review
- Semi-in vitro detection of MgScientific reports · 2022Article
- Cancer Stem-Like Phenotype of Mitochondria Dysfunctional Hep3B Hepatocellular Carcinoma Cell Line.Cells · 2021Article
- Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.The Journal of cell biology · 2021Article
- Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6ρ0 cells.Molecular medicine reports · 2021Article
- Article
- Phosphorylation and chromatin tethering prevent cGAS activation during mitosis.Science (New York, N.Y.) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Here, we document that persistent mitochondria DNA (mtDNA) damage due to mitochondrial overexpression of the Y147A mutant uracil-N-glycosylase as well as mitochondrial overexpression of bacterial Exonuclease III or Herpes Simplex Virus protein UL12.5M185 can induce a complete loss of mtDNA (ρ0 phenotype) without compromising the viability of cells cultured in media supplemented with uridine and pyruvate. Furthermore, we use these observations to develop rapid, sequence-independent methods for the elimination of mtDNA, and demonstrate utility of these methods for generating ρ0 cells of human, mouse and rat origin. We also demonstrate that ρ0 cells generated by each of these three methods can serve as recipients of mtDNA in fusions with enucleated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.