ArticleNature biotechnology2016
DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.
Article in Nature biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 78 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
78 citing papers in PubMed.
- Distinct Cleavage Trajectories Mediated by DNA and RNA Substrate in Argonaute From Methanocaldococcus Fervens.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Application ofJournal of bacteriology · 2025Article
- The nucleic acid reactions on the nanomaterials surface for biomedicine.Journal of nanobiotechnology · 2025Review
- Emerging Gene-editing nano-therapeutics for Cancer.Heliyon · 2024Review
- Efficient manipulation of gene expression using Natronobacterium gregoryi Argonaute in zebrafish.BMC biology · 2023Article
- Prokaryotic Argonaute Proteins as a Tool for Biotechnology.Molecular biology · 2022Article
- Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security.International journal of molecular sciences · 2021Review
- Argonaute proteins: structures and their endonuclease activity.Molecular biology reports · 2021Review
- Article
- Insertion/deletion-activated frame-shift fluorescence protein is a sensitive reporter for genomic DNA editing.BMC genomics · 2019Article
- DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute.Nucleic acids research · 2019Article
- CRISPR-based genome editing in wheat: a comprehensive review and future prospects.Molecular biology reports · 2019Review
- Reboot ethics governance in China.Nature · 2019Article
- The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria.Nucleic acids research · 2019Article
- DNA interference by a mesophilic Argonaute protein, CbcAgo.F1000Research · 2019Article
- Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants.Frontiers in plant science · 2019Review
- HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 orAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Zebrafish Embryonic Slow Muscle Is a Rapid System for Genetic Analysis of Sarcomere Organization by CRISPR/Cas9, but Not NgAgo.Marine biotechnology (New York, N.Y.) · 2018Article
- Effects of HPV Pseudotype Virus in Cutting E6 Gene Selectively in SiHa Cells.Current medical science · 2018Article
- Key Anti-Fibrosis Associated Long Noncoding RNAs Identified in Human Hepatic Stellate Cell via Transcriptome Sequencing Analysis.International journal of molecular sciences · 2018Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
- Expression of concern · 2016-11-28Concerns/Issues about Data · Objections by Third Party ·
- Retraction · 2017-07-26Concerns/Issues about Data · Objections by Third Party · Results Not Reproducible ·
- Commented on by
- Commented on by
- Commented on by
- Expression of concern
- Retracted
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ∼24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.
Indexed as
Identifiers
27136078What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.