ArticlePloS one2016
1,25-Dihydroxyvitamin-D3 Induces Avian β-Defensin Gene Expression in Chickens.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- Further Evaluation of 1,25(OH)Animals : an open access journal from MDPI · 2026Article
- Oral vitamin DPoultry science · 2026Article
- Growth Performance and Gut Health of Cold-Stressed Broilers in Response to Supplementation with a Combination of Sodium Butyrate and Vitamin D3.Animals : an open access journal from MDPI · 2025Article
- Differential Expression of Key Immune Markers in the Intestinal Tract of Developing Chick Embryos.Veterinary sciences · 2025Article
- Mechanisms and regulation of defensins in host defense.Signal transduction and targeted therapy · 2023Review
- Evaluating protective effects of botanicals under inflammation and oxidative stress in chicken apical-out enteroids.Poultry science · 2023Article
- Effects of the Vitamin D3 on Alleviating the Oxidative Stress Induced by Diquat in Wenchang Chickens.Animals : an open access journal from MDPI · 2023Article
- Interactive effect of dietary levels of calcium and 25-hydroxy vitamin D3 on the performance, serum biochemical concentration and digestibility of laying hens from 61 to 70 weeks of age.Animal bioscience · 2022Article
- High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression.Antibiotics (Basel, Switzerland) · 2022Article
- Toll-Like Receptors Signaling Pathway of Quercetin Regulating Avian Beta-Defensin in the Ileum of Broilers.Frontiers in cell and developmental biology · 2022Article
- Incubation Temperature and Lighting: Effect on Embryonic Development, Post-Hatch Growth, and Adaptive Response.Frontiers in physiology · 2022Review
- Immunomodulatory Effects of Dietary Phosphorus and Calcium in Two Strains of Laying Hens.Animals : an open access journal from MDPI · 2021Article
- Synergistic Induction of Chicken Antimicrobial Host Defense Peptide Gene Expression by Butyrate and Sugars.Frontiers in microbiology · 2021Article
- Peptidoglycan derived fromInnate immunity · 2020Article
- Effects of Toll-like Receptor Ligands on the Expression of Proinflammatory Cytokines and AvianThe journal of poultry science · 2020Article
- Dietary Nutrients Mediate Intestinal Host Defense Peptide Expression.Advances in nutrition (Bethesda, Md.) · 2020Review
- Calcium and Cholecalciferol Levels in Late-Phase Laying Hens: Effects on Productive Traits, Egg Quality, Blood Biochemistry, and Immune Responses.Frontiers in veterinary science · 2020Article
- Examination of the Expression of Immunity Genes and Bacterial Profiles in the Caecum of Growing Chickens Infected withAnimals : an open access journal from MDPI · 2019Article
- Human Antimicrobial RNases Inhibit Intracellular Bacterial Growth and Induce Autophagy in Mycobacteria-Infected Macrophages.Frontiers in immunology · 2019Article
- The potential for immunoglobulins and host defense peptides (HDPs) to reduce the use of antibiotics in animal production.Veterinary research · 2018Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Host defense peptides (HDPs) play a critical role in innate immunity. Specific modulation of endogenous HDP synthesis by dietary compounds has been regarded as a novel approach to boost immunity and disease resistance in animal production. 1,25-dihydroxy vitamin D3 (1,25D3) is well known as a powerful HDP inducer in humans, but limited information about the effect of 1,25D3 on HDPs in poultry is available. Here, we sought to examine whether 1,25D3 could stimulate avian β-defensin (AvBD) expression in chickens. We used chicken embryo intestinal epithelial cells (CEIEPCs) and peripheral blood mononuclear cells (PBMCs) to study the effect of 1,25D3 on the expression of AvBDs. We observed that 1,25D3 is able to up-regulate the expression of several AvBDs in CEIEPCs and PBMCs, whereas it increased the amounts of AvBD4 mRNA in CEIEPCs only in the presence of lipopolysaccharide (LPS). On the other hand, LPS treatment not only inhibited the expression of CYP24A1 but also altered the expression pattern of VDR in CEIEPCs. Furthermore, AvBDs were not directly regulated by 1,25D3, as cycloheximide completely blocked 1,25D3-induced expression of AvBDs. Our observations suggest that 1,25D3 is capable of inducing AvBD gene expression and is a potential antibiotic alternative through augmentation of host innate immunity as well as disease control in chickens.
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