ArticleCell systems2016
Optogenetic Control of Calcium Oscillation Waveform Defines NFAT as an Integrator of Calcium Load.
Article in Cell systems, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Virus-like antigen display delivers a stand-alone danger signal through the BCR that circumvents tolerance.bioRxiv : the preprint server for biology · 2026Article
- A molecular systems perspective on calcium oscillations beyond ion fluxes.Current opinion in cell biology · 2025Review
- Isogenic iPSC-derivedFrontiers in neuroscience · 2025Article
- Optogenetics in Pancreatic Islets: Actuators and Effects.Diabetes · 2024Review
- Ultrasound-mediated spatial and temporal control of engineered cells in vivo.Nature communications · 2024Article
- Transient frequency preference responses in cell signaling systems.NPJ systems biology and applications · 2024Article
- Co-imaging of RelA and c-Rel reveals features of NF-κB signaling for ligand discrimination.Cell reports · 2024Article
- Cardiac optogenetics: shining light on signaling pathways.Pflugers Archiv : European journal of physiology · 2023Review
- High-throughput feedback-enabled optogenetic stimulation and spectroscopy in microwell plates.Communications biology · 2023Article
- Optogenetically engineered Ca2+ oscillation-mediated DRP1 activation promotes mitochondrial fission and cell death.Journal of cell science · 2023Article
- Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics.iScience · 2023Article
- LITOS: a versatile LED illumination tool for optogenetic stimulation.Scientific reports · 2022Article
- Towards 'end-to-end' analysis and understanding of biological timecourse data.The Biochemical journal · 2022Article
- Dynamic changes in β-cell [CaMolecular metabolism · 2022Article
- Inhibition of Noncanonical CaNeurochemical research · 2022Article
- TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes.Frontiers in physiology · 2022Article
- Platforms for Optogenetic Stimulation and Feedback Control.Frontiers in bioengineering and biotechnology · 2022Review
- Deciphering Molecular Mechanisms and Intervening in Physiological and Pathophysiological Processes of CaCells · 2021Review
- Progress and challenges in understanding the regulation and function of p53 dynamics.Biochemical Society transactions · 2021Review
- Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems.Annual review of biomedical engineering · 2021Review
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2 authors.
Funding
Abstract
It is known that the calcium-dependent transcription factor NFAT initiates transcription in response to pulsatile loads of calcium signal. However, the relative contributions of calcium oscillation frequency, amplitude, and duty cycle to transcriptional activity remain unclear. Here, we engineer HeLa cells to permit optogenetic control of intracellular calcium concentration using programmable LED arrays. This approach allows us to generate calcium oscillations of constant peak amplitude, in which frequency is varied while holding duty cycle constant, or vice versa. Using this setup and mathematical modeling, we show that NFAT transcriptional activity depends more on duty cycle, defined as the proportion of the integrated calcium concentration over the oscillation period, than on frequency alone. This demonstrates that NFAT acts primarily as a signal integrator of cumulative load rather than a frequency-selective decoder. This approach resolves a fundamental question in calcium encoding and demonstrates the value of optogenetics for isolating individual dynamical components of larger signaling behaviors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.