ArticlePlants (Basel, Switzerland)2014
Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis.
Article in Plants (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 36 citations in OpenAlex.
- Identification of a novel link connecting indole-3-acetamide with abscisic acid biosynthesis and signaling.The New phytologist · 2026Article
- Analyzing Plant Low-Molecular-Weight Polar Metabolites: A GC-MS Approach.Plants (Basel, Switzerland) · 2026Review
- Auxin accumulation in cereals after infection by Fusarium graminearum: putative biosynthetic pathways and preferences.Stress biology · 2026Review
- Influence of Tryptophan Metabolism on the Protective Effect of Weissella paramesenteroides WpK4 in a Murine Model of Chemotherapy-Induced Intestinal Mucositis.Probiotics and antimicrobial proteins · 2025Article
- Brassinosteroid Synthesis and Perception Differently Regulate Phytohormone Networks inInternational journal of molecular sciences · 2025Article
- Do Opposites Attract? Auxin-Abscisic Acid Crosstalk: New Perspectives.International journal of molecular sciences · 2023Review
- The Indole-3-Acetamide-InducedFrontiers in plant science · 2022Article
- Soybean Leaf Proteomic Profile Influenced by Rhizobacteria Under Optimal and Salt Stress Conditions.Frontiers in plant science · 2022Article
- Plants Metabolome Study: Emerging Tools and Techniques.Plants (Basel, Switzerland) · 2021Review
- Beyond the Usual Suspects: Physiological Roles of the Arabidopsis Amidase Signature (AS) Superfamily Members in Plant Growth Processes and Stress Responses.Biomolecules · 2021Review
- Function of histone H2B monoubiquitination in transcriptional regulation of auxin biosynthesis in Arabidopsis.Communications biology · 2021Article
- GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis.Plant & cell physiology · 2020Article
- A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.).BMC plant biology · 2019Article
- Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls.Plant & cell physiology · 2019Article
- Identification of Two Auxin-Regulated Potassium Transporters Involved in Seed Maturation.International journal of molecular sciences · 2018Article
- A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings.PloS one · 2018Article
- Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases inFrontiers in plant science · 2018Article
- Control of Endogenous Auxin Levels in Plant Root Development.International journal of molecular sciences · 2017Review
- Metabolomic compounds identified in Piriformospora indica-colonized Chinese cabbage roots delineate symbiotic functions of the interaction.Scientific reports · 2017Article
- Possible Interactions between the Biosynthetic Pathways of Indole Glucosinolate and Auxin.Frontiers in plant science · 2017Review
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amidases [EC 3.5.1.4] capable of converting indole-3-acetamide (IAM) into the major plant growth hormone indole-3-acetic acid (IAA) are assumed to be involved in auxin de novo biosynthesis. With the emerging amount of genomics data, it was possible to identify over forty proteins with substantial homology to the already characterized amidases from Arabidopsis and tobacco. The observed high conservation of amidase-like proteins throughout the plant kingdom may suggest an important role of theses enzymes in plant development. Here, we report cloning and functional analysis of four, thus far, uncharacterized plant amidases from Oryza sativa, Sorghum bicolor, Medicago truncatula, and Populus trichocarpa. Intriguingly, we were able to demonstrate that the examined amidases are also capable of converting phenyl-2-acetamide (PAM) into phenyl-2-acetic acid (PAA), an auxin endogenous to several plant species including Arabidopsis. Furthermore, we compared the subcellular localization of the enzymes to that of Arabidopsis AMI1, providing further evidence for similar enzymatic functions. Our results point to the presence of a presumably conserved pathway of auxin biosynthesis via IAM, as amidases, both of monocot, and dicot origins, were analyzed.
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