ArticlePloS one2016
Microbiome of Deep Dentinal Caries Lesions in Teeth with Symptomatic Irreversible Pulpitis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 89 citations in OpenAlex.
- Meta-Analysis Using NGS Data: TheFrontiers in oral health · 2021Pooled it
- The circ_0042103/TAF15/NER axis regulates inflammation and DNA damage in pulpitis.Stem cell research & therapy · 2026Article
- Investigating oral microbiome profiles of children with cleft lip and palate in different states of caries.Journal of oral microbiology · 2026Article
- Pulpitis as a microbial disease: single-cell insights into host responses and diagnostic biomarkers for vital pulp therapy.Journal of oral microbiology · 2026Review
- On the necessity of complete caries excavation: a narrative review.Head & face medicine · 2025Review
- Microbiota of Healthy Dental Pulp Under the Omics Loupe.International journal of molecular sciences · 2025Article
- Oral microbiota in an aging Swedish population with high dental disease burden: an observational registry-based study.Frontiers in oral health · 2025Article
- Altered salivary microbiota associated with high-sugar beverage consumption.Scientific reports · 2024Article
- The Evolving Microbiome of Dental Caries.Microorganisms · 2024Review
- SrCuSiAdvanced healthcare materials · 2023Article
- Regulation of caries-induced pulp inflammation by NLRP3 inflammasome: A laboratory-based investigation.International endodontic journal · 2023Article
- Single-cell atlas of dental pulp stem cells exposed to the oral bacteriaFrontiers in cell and developmental biology · 2023Article
- Paecilomyces variotti in deep dental caries.Journal of clinical and experimental dentistry · 2022Article
- Alternative Antibiotics in Dentistry: Antimicrobial Peptides.Pharmaceutics · 2022Review
- Article
- Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis.Microorganisms · 2021Article
- Cariogenic and oral health taxa in the oral cavity among children and adults: A scoping review.Archives of oral biology · 2021Article
- Low-Abundant Microorganisms: The Human Microbiome's Dark Matter, a Scoping Review.Frontiers in cellular and infection microbiology · 2021Article
- Proteome Analysis of Molecular Events in Oral Pathogenesis and Virus: A Review with a Particular Focus on Periodontitis.International journal of molecular sciences · 2020Review
- Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study.BMC oral health · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study used a next-generation sequencing approach to identify the bacterial taxa occurring in the advanced front of caries biofilms associated with pulp exposure and irreversible pulpitis. Samples were taken from the deepest layer of dentinal caries lesions associated with pulp exposure in 10 teeth diagnosed with symptomatic irreversible pulpitis. DNA was extracted and the microbiome was characterized on the basis of the V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device. Bacterial taxa were mapped to 14 phyla and 101 genera composed by 706 different OTUs. Three phyla accounted for approximately 98% of the sequences: Firmicutes, Actinobacteria and Proteobacteria. These phyla were also the ones with most representatives at the species level. Firmicutes was the most abundant phylum in 9/10 samples. As for genera, Lactobacillus accounted for 42.3% of the sequences, followed by Olsenella (13.7%), Pseudoramibacter (10.7%) and Streptococcus (5.5%). Half of the samples were heavily dominated by Lactobacillus, while in the other half lactobacilli were in very low abundance and the most dominant genera were Pseudoramibacter, Olsenella, Streptococcus, and Stenotrophomonas. High bacterial diversity occurred in deep dentinal caries lesions associated with symptomatic irreversible pulpitis. The microbiome could be classified according to the relative abundance of Lactobacillus. Except for Lactobacillus species, most of the highly prevalent and abundant bacterial taxa identified in this study have been commonly detected in infected root canals. The detected taxa can be regarded as candidate pathogens for irreversible pulpitis and possibly the pioneers in pulp invasion to initiate endodontic infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.