Evidence map›Paper›PMID 27134874›Full record

ReviewJournal of clinical and diagnostic research : JCDR2016

Cell Culture, Technology: Enhancing the Culture of Diagnosing Human Diseases.

Shuaibu Abdullahi Hudu, Ahmed Subeh Alshrari, Ahmad Syahida, Zamberi Sekawi

Abstract readReview
In one paragraph

Review in Journal of clinical and diagnostic research : JCDR, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Integrating metagenomics and metatranscriptomics intoThe Journal of general virology · 2026
    Review
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Cancer Cell Culture: The Basics and Two-Dimensional Cultures.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  9. Viral culture and immunofluorescence for the detection of SARS-CoV-2 infectivity in RT-PCR positive respiratory samples.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2022
    Article
  10. Article
  11. Review
  12. Article
  13. From Donor to the Lab: A Fascinating Journey of Primary Cell Lines.Frontiers in cell and developmental biology · 2021
    Review
  14. Advances in airborne microorganisms detection using biosensors: A critical review.Frontiers of environmental science & engineering · 2021
    Review
  15. AFM-Based Correlative Microscopy Illuminates Human Pathogens.Frontiers in cellular and infection microbiology · 2021
    Review
  16. Article
  17. Review
  18. Article
  19. A RT-PCR assay for the detection of coronaviruses from four genera.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shuaibu Abdullahi HuduFaculty, Department of Medical Microbiology and Parasitology, Faculty of Basic Medical Sciences, College of Health Sciences, Usmanu Danfodiyo University , Sokoto, Sokoto State, Nigeria .
Ahmed Subeh AlshrariFaculty, Department of Basic Health Sciences, Faculty of Pharmacy, Northern Border Universiti , Rafha, Saudi Arabia .
Ahmad SyahidaProfessor, Department of Biochemistry, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia . UPM Serdang, Selangor Darul Ehsan, Malaysia .
Zamberi SekawiProfessor, Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia . UPM Serdang, Selangor Darul Ehsan, Malaysia .

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell culture involves a complex of processes of cell isolation from their natural environment (in vivo) and subsequent growth in a controlled environmental artificial condition (in vitro). Cells from specific tissues or organs are cultured as short term or established cell lines which are widely used for research and diagnosis, most specially in the aspect of viral infection, because pathogenic viral isolation depends on the availability of permissible cell cultures. Cell culture provides the required setting for the detection and identification of numerous pathogens of humans, which is achieved via virus isolation in the cell culture as the "gold standard" for virus discovery. In this review, we summarized the views of researchers on the current role of cell culture technology in the diagnosis of human diseases. The technological advancement of recent years, starting with monoclonal antibody development to molecular techniques, provides an important approach for detecting presence of viral infection. They are also used as a baseline for establishing rapid tests for newly discovered pathogens. A combination of virus isolation in cell culture and molecular methods is still critical in identifying viruses that were previously unrecognized. Therefore, cell culture should be considered as a fundamental procedure in identifying suspected infectious viral agent.

Indexed as

Pathogen discoveryRecombinant proteinTransgenic cell lineViral isolation

Identifiers

PMID27134874
PMCPMC4843260

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.