ArticleJournal of biological engineering2016
Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9.
Article in Journal of biological engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Genetic landscape of an in vivo protein interactome.Nature genetics · 2026Article
- High-throughput approaches to functional characterization of genetic variation in yeast.Current opinion in genetics & development · 2022Review
- Asymmetrical dose responses shape the evolutionary trade-off between antifungal resistance and nutrient use.Nature ecology & evolution · 2022Article
- Deep Mutational Scanning of Protein-Protein Interactions Between Partners Expressed from Their Endogenous Loci In Vivo.Methods in molecular biology (Clifton, N.J.) · 2022Article
- AddTag, a two-step approach with supporting software package that facilitates CRISPR/Cas-mediated precision genome editing.G3 (Bethesda, Md.) · 2021Article
- Stress tolerance enhancement via SPT15 base editing in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Protein context shapes the specificity of SH3 domain-mediated interactions in vivo.Nature communications · 2021Article
- The evolution of coexistence from competition in experimental co-cultures of Escherichia coli and Saccharomyces cerevisiae.The ISME journal · 2021Article
- Strategies for High-Efficiency Mutation Using the CRISPR/Cas System.Frontiers in cell and developmental biology · 2021Review
- Precise Replacement ofG3 (Bethesda, Md.) · 2020Article
- CRISPR-Cas9 Genome Editing Tool for the Production of Industrial Biopharmaceuticals.Molecular biotechnology · 2020Review
- Development and Application of CRISPR/Cas in Microbial Biotechnology.Frontiers in bioengineering and biotechnology · 2020Review
- CRISPR/Cas9 Systems for the Development ofFrontiers in bioengineering and biotechnology · 2020Review
- A novel method to investigate the effects of gene mutations at the cellular level using a dual expression lentiviral vector.Bioscience reports · 2019Article
- Yeast genetic interaction screens in the age of CRISPR/Cas.Current genetics · 2019Review
- Improved bioethanol production using CRISPR/Cas9 to disrupt the ADH2 gene in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2018Article
- In vivo base editing of post-mitotic sensory cells.Nature communications · 2018Article
- Determinants of selection in yeast evolved by genome shuffling.Biotechnology for biofuels · 2018Article
- Yeast Still a Beast: Diverse Applications of CRISPR/Cas Editing Technology inThe Yale journal of biology and medicine · 2017Review
- A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR assisted homology directed repair enables the introduction of virtually any modification to the Saccharomyces cerevisiae genome. Of obvious interest is the marker-free and seamless introduction of point mutations. To fulfill this promise, a strategy that effects single nucleotide changes while preventing repeated recognition and cutting by the gRNA/Cas9 complex is needed. We demonstrate a two-step method to introduce point mutations at 17 positions in the S. cerevisiae genome. We show the general applicability of the method, enabling the seamless introduction of single nucleotide changes at any location, including essential genes and non-coding regions. We also show a quantifiable phenotype for a point mutation introduced in gene GSH1. The ease and wide applicability of this general method, combined with the demonstration of its feasibility will enable genome editing at an unprecedented level of detail in yeast and other organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.