ArticlePlant biotechnology journal2016
Identification of a Chlamydomonas plastidial 2-lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 88 citations in OpenAlex.
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- A Plasma Membrane-Located Lysophosphatidic Acid Acyltransferase in Microalga Myrmecia incisa Prefers Arachidonic Acid-CoA to Produce Glycerolipids.Applied biochemistry and biotechnology · 2026Article
- Organelle-Targeted Multi-Enzyme Engineering Enables Medium-Chain Fatty Acid Production inJournal of microbiology and biotechnology · 2026Article
- Metabolic Engineering of Microalgae for Biofuel Production.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives.Marine drugs · 2024Review
- Evaluation of Cellular Responses byMicroorganisms · 2024Article
- Microalgal glycerol-3-phosphate acyltransferase role in galactolipids and high-value storage lipid biosynthesis.Plant physiology · 2023Article
- Lysophosphatidic acid acyltransferase 2 and 5 commonly, but differently, promote seed oil accumulation in Brassica napus.Biotechnology for biofuels and bioproducts · 2022Article
- Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions.Biotechnology for biofuels and bioproducts · 2022Article
- Diacylglyceryl-N,N,N-trimethylhomoserine-dependent lipid remodeling in a green alga, Chlorella kessleri.Communications biology · 2022Article
- Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture.Molecules (Basel, Switzerland) · 2021Review
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green AlgaPlants (Basel, Switzerland) · 2021Article
- A Novel Bifunctional Wax Ester Synthase Involved in Early Triacylglycerol Accumulation in Unicellular Green MicroalgaFrontiers in bioengineering and biotechnology · 2021Article
- Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria.Advances in experimental medicine and biology · 2021Article
- Article
- CrABCA2 Facilitates Triacylglycerol Accumulation inMolecules and cells · 2020Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity inBiotechnology for biofuels · 2020Article
- Identification and Biotechnical Potential of a Gcn5-Related N-Acetyltransferase Gene in Enhancing Microalgal Biomass and Starch Production.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite a strong interest in microalgal oil production, our understanding of the biosynthetic pathways that produce algal lipids and the genes involved in the biosynthetic processes remains incomplete. Here, we report that Chlamydomonas reinhardtii Cre09.g398289 encodes a plastid-targeted 2-lysophosphatidic acid acyltransferase (CrLPAAT1) that acylates the sn-2 position of a 2-lysophosphatidic acid to form phosphatidic acid, the first common precursor of membrane and storage lipids. In vitro enzyme assays showed that CrLPAAT1 prefers 16:0-CoA to 18:1-CoA as an acyl donor. Fluorescent protein-tagged CrLPAAT1 was localized to the plastid membrane in C. reinhardtii cells. Furthermore, expression of CrLPAAT1 in plastids led to a > 20% increase in oil content under nitrogen-deficient conditions. Taken together, these results demonstrate that CrLPAAT1 is an authentic plastid-targeted LPAAT in C. reinhardtii, and that it may be used as a molecular tool to genetically increase oil content in microalgae.
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