ArticleNucleic acids research2016
Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- The association between rs16917496 T/C polymorphism ofBioscience reports · 2018Pooled it
- KMT5A maintains muscle stem cell quiescence through epigenetic regulation of Notch signaling.iScience · 2026Article
- Role of histone methylation in insect development: KMT5A regulates ecdysteroid biosynthesis during metamorphosis of Tribolium castaneum.Insect biochemistry and molecular biology · 2025Article
- Lysine Methylation and Histone Modifications during Cold Stress of Insects: Freeze-TolerantInsects · 2024Article
- Article
- A dual role for the chromatin reader ORCA/LRWD1 in targeting the origin recognition complex to chromatin.The EMBO journal · 2023Article
- Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall FlyInternational journal of molecular sciences · 2023Article
- Phosphorylated histone variant γH2Av is associated with chromatin insulators in Drosophila.PLoS genetics · 2022Article
- Distinct developmental phenotypes result from mutation of Set8/KMT5A and histone H4 lysine 20 in Drosophila melanogaster.Genetics · 2022Article
- The Role of Histone Lysine Methylation in the Response of Mammalian Cells to Ionizing Radiation.Frontiers in genetics · 2021Review
- Methylation of histone 4's lysine 20: a critical analysis of the state of the field.Physiological genomics · 2021Review
- H4K20 monomethylation inhibition causes loss of genomic integrity in mouse preimplantation embryos.The Journal of reproduction and development · 2020Article
- Meiotic gatekeeper STRA8 regulates cell cycle by interacting with SETD8 during spermatogenesis.Journal of cellular and molecular medicine · 2020Article
- Review
- Cell-cycle regulation of non-enzymatic functions of the Drosophila methyltransferase PR-Set7.Nucleic acids research · 2018Article
- Histone methylation in DNA repair and clinical practice: new findings during the past 5-years.Journal of Cancer · 2018Review
- DNA replication through a chromatin environment.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication.The EMBO journal · 2017Article
- DNA Replication Control DuringGenetics · 2017Review
- Writing, erasing and reading histone lysine methylations.Experimental & molecular medicine · 2017Review
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
The methylation state of lysine 20 on histone H4 (H4K20) has been linked to chromatin compaction, transcription, DNA repair and DNA replication. Monomethylation of H4K20 (H4K20me1) is mediated by the cell cycle-regulated histone methyltransferase PR-Set7. PR-Set7 depletion in mammalian cells results in defective S phase progression and the accumulation of DNA damage, which has been partially attributed to defects in origin selection and activation. However, these studies were limited to only a handful of mammalian origins, and it remains unclear how PR-Set7 and H4K20 methylation impact the replication program on a genomic scale. We employed genetic, cytological, and genomic approaches to better understand the role of PR-Set7 and H4K20 methylation in regulating DNA replication and genome stability in Drosophila cells. We find that deregulation of H4K20 methylation had no impact on origin activation throughout the genome. Instead, depletion of PR-Set7 and loss of H4K20me1 results in the accumulation of DNA damage and an ATR-dependent cell cycle arrest. Coincident with the ATR-dependent cell cycle arrest, we find increased DNA damage that is specifically limited to late replicating regions of the Drosophila genome, suggesting that PR-Set7-mediated monomethylation of H4K20 is critical for maintaining the genomic integrity of late replicating domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.