Evidence map›Paper›PMID 27130910›Full record

ArticleIranian biomedical journal2016

Melatonin Pretreatment Enhances the Homing of Bone Marrow-derived Mesenchymal Stem Cells Following Transplantation in a Rat Model of Liver Fibrosis.

Keywan Mortezaee, Parichehr Pasbakhsh, Iraj Ragerdi Kashani, Fatemeh Sabbaghziarani, Ameneh Omidi, Adib Zendedel, Soudabeh Ghasemi, Ahmad Reza Dehpour

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In one paragraph

Article in Iranian biomedical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed, 4 pooled it
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 4 syntheses or guidelines pooled it, 54 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Keywan MortezaeeDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Parichehr PasbakhshDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iraj Ragerdi KashaniDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Fatemeh SabbaghziaraniDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ameneh OmidiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Adib ZendedelInstitute of Neuroanatomy, School of Medicine, RWTH Aachen University, 52074 Aachen, Germany.
Soudabeh GhasemiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ahmad Reza DehpourDepartment of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Tehran University of Medical Sciences · IRRWTH Aachen University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone marrow-derived mesenchymal stem cells (BMMSCs) transplantation has been considered as a promising milestone in liver fibrosis treatment. However, low amounts of homing are a major obstacle. We aimed to investigate the role of melatonin pretreatment in BMMSC homing into experimental liver fibrosis.

methodsBMMSCs were obtained, grown, propagated and preconditioned with 5 µM melatonin and analyzed for multipotency and immunophenotypic features at passage three. The cells were labelled with CM-Dil and infused into the rats received the i.p. injection of carbon tetrachloride (CCl4) for five weeks to induce liver fibrosis. Animals were divided into two groups: One group received BMMSCs, whereas the other group received melatonin-pretreated BMMSCs (MT-BMMSCs). After cell injection at 72 h, animals were sacrificed, and the liver tissues were assessed for further evaluations: fibrosis using Masson's trichrome and hematoxylin and eosin staining and homing using fluorescent microscopy and flow cytometry.

resultsBMMSCs and MT-BMMSCs expressed a high level of CD44 but low levels of CD11b, CD45 and CD34 (for all P≤0.05) and were able to differentiate into adipocytes and Schwann cells. CCl4 induction resulted in extensive collagen deposition, tissue disruption and fatty accumulation with no obvious difference between the two groups. There was a significant increase in homing of MT-BMMSCs in both florescent microscopy (P≤0.001) and flow cytometry (P≤0.01) assays, as compared with non-treated BMMSCs.

conclusionThis study indicates the improved homing potential of BMMSCs in pretreatment with melatonin. Therefore, this strategy may represent an applied approach for improving the stem cell therapy of liver fibrosis.

Indexed as

AdipocytesAdipogenesisAnimalsAntigens, CD34Bone Marrow CellsCarbon TetrachlorideCD11b AntigenCell- and Tissue-Based TherapyCell MovementCells, CulturedCell TransdifferentiationHyaluronan ReceptorsLeukocyte Common AntigensLiverLiver CirrhosisMaleAntigens, CD34Carbon TetrachlorideCD11b AntigenHyaluronan ReceptorsLeukocyte Common AntigensMelatoninPtprc protein, ratBone marrowMelatoninMesenchymal stem cells

Identifiers

PMID27130910
PMCPMC4983675
OpenAlexW2416404014

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.