ArticleIn vitro cellular & developmental biology. Animal2016
Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods.
Article in In vitro cellular & developmental biology. Animal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Development of an Eye Irritation Test Method Using an In-House Fabrication of a Reconstructed Human Cornea-like Epithelium Model for Eye Hazard Identification.Bioengineering (Basel, Switzerland) · 2024Article
- Function of mitochondrial cytochrome c oxidase is enhanced in human lens epithelial cells at high temperatures.Molecular medicine reports · 2023Article
- Morphological comparison between three-dimensional structure of immortalized human lens epithelial cells and Soemmering's ring.Medical molecular morphology · 2021Article
- Article
- Oral intake of α‑glucosyl‑hesperidin ameliorates selenite‑induced cataract formation.Molecular medicine reports · 2020Article
- Establishment of primary bovine intestinal epithelial cell culture and clone method.In vitro cellular & developmental biology. Animal · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
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