ArticleCell and tissue banking2016
Cartilage storage at 4 °C with regular culture medium replacement benefits chondrocyte viability of osteochondral grafts in vitro.
Article in Cell and tissue banking, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 17 citations in OpenAlex.
- Evaluation of allogenic nasal cartilage storage media in terms of biosafety and viability.Cell and tissue banking · 2026Article
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- Novel Allograft in the Load-Bearing Portion of the Femoral Head.Ochsner journal · 2024Article
- Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Comparative Study Using Fluorescent Confocal Microscopy and Flow Cytometry to Evaluate Chondrocyte Viability in Human Osteochondral Allografts.Bioengineering (Basel, Switzerland) · 2022Article
- A comprehensive tool box for large animal studies of intervertebral disc degeneration.JOR spine · 2021Article
- Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells.International journal of molecular sciences · 2020Article
- Article
- Effects of Rolling-Sliding Mechanical Stimulation on Cartilage PreservedCellular and molecular bioengineering · 2019Article
- [Non-cryoconserving storage strategies for fresh osteochondral allografts].Der Unfallchirurg · 2017Review
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Authors and funding
7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Maintenance of articular cartilage allografts in culture media is a common method of tissue storage; however, the technical parameters of graft storage remain controversial. In this study, we examined the optimal temperature and culture medium exchange rate for the storage of osteochondral allografts in vitro. Cylindrical osteochondral grafts (n = 120), harvested from the talar joint surface of ten Boer goats, were randomly classified into four groups and stored under the following conditions: Group A1 was maintained at 4 °C in culture medium that was refreshed every 2 days; Group A2 was maintained at 4 °C in the same culture medium, without refreshing; Group B1, was maintained at 37 °C in culture medium that was refreshed every 2 days; Group B2, was maintained at 37 °C in the same culture medium, without refreshing. Chondrocyte viability in the grafts was determined by ethidium bromide/fluorescein diacetate staining on days 7, 21, and 35. Proteoglycan content was measured by Safranin-O staining. Group A1 exhibited the highest chondrocyte survival rates of 90.88 %, 88.31 % and 78.69 % on days 7, 21, and 35, respectively. Safranin O staining revealed no significant differences between groups on days 21 and 35. These results suggest that storage of osteochondral grafts at 4 °C with regular culture medium replacement should be highly suitable for clinical application.
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