ArticleJournal of experimental botany2016
Large variation in the Rubisco kinetics of diatoms reveals diversity among their carbon-concentrating mechanisms.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 203 citations in OpenAlex.
- The fitness landscape of a form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance.Science advances · 2026Article
- Rubisco kinetic diversity and effectiveness of CO2-concentrating mechanisms in aquatic photosynthetic organisms.Plant physiology · 2026Review
- Diversity in Rubisco kinetics and CO2-concentrating mechanisms in Cyanobacteria.Plant physiology · 2026Article
- Functional characterization of bicarbonate transporters from the cyanobacterial SbtA2 family and subsequent expression in tobacco.Journal of experimental botany · 2026Article
- Engineering and Biological Mechanisms of Microalgal COMicroorganisms · 2026Review
- Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms from diverse species show differences in oligomeric structure, thermal stability, and activity.European biophysics journal : EBJ · 2025Article
- A SynBio explosion: a whole new world for Rubisco engineering.Journal of experimental botany · 2025Article
- Chemoautotrophy in subzero environments and the potential for cold-adapted Rubisco.Applied and environmental microbiology · 2025Article
- Inorganic Carbon Acquisition and Photosynthetic Metabolism in Marine Photoautotrophs: A Summary.Plants (Basel, Switzerland) · 2025Review
- Review
- Inorganic carbon enrichment does not increase production of polyunsaturated aldehydes in a pelagic and benthic diatom.PloS one · 2025Article
- Regulation of Microalgal Photosynthetic Electron Transfer.Plants (Basel, Switzerland) · 2024Review
- Mechanistic constraints on the trade-off between photosynthesis and respiration in response to warming.Science advances · 2023Article
- Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth.Nature plants · 2023Article
- Singular adaptations in the carbon assimilation mechanism of the polyextremophile cyanobacterium Chroococcidiopsis thermalis.Photosynthesis research · 2023Article
- Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2023Article
- The carbon-concentrating mechanism of the extremophilic red microalga Cyanidioschyzon merolae.Photosynthesis research · 2023Article
- The trajectory in catalytic evolution of Rubisco in Posidonia seagrass species differs from terrestrial plants.Plant physiology · 2023Article
- Red Rubiscos and opportunities for engineering green plants.Journal of experimental botany · 2023Review
10 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
While marine phytoplankton rival plants in their contribution to global primary productivity, our understanding of their photosynthesis remains rudimentary. In particular, the kinetic diversity of the CO2-fixing enzyme, Rubisco, in phytoplankton remains unknown. Here we quantify the maximum rates of carboxylation (k cat (c)), oxygenation (k cat (o)), Michaelis constants (K m) for CO2 (K C) and O2 (K O), and specificity for CO2 over O2 (SC/O) for Form I Rubisco from 11 diatom species. Diatom Rubisco shows greater variation in K C (23-68 µM), SC/O (57-116mol mol(-1)), and K O (413-2032 µM) relative to plant and algal Rubisco. The broad range of K C values mostly exceed those of C4 plant Rubisco, suggesting that the strength of the carbon-concentrating mechanism (CCM) in diatoms is more diverse, and more effective than previously predicted. The measured k cat (c) for each diatom Rubisco showed less variation (2.1-3.7s(-1)), thus averting the canonical trade-off typically observed between K C and k cat (c) for plant Form I Rubisco. Uniquely, a negative relationship between K C and cellular Rubisco content was found, suggesting variation among diatom species in how they allocate their limited cellular resources between Rubisco synthesis and their CCM. The activation status of Rubisco in each diatom was low, indicating a requirement for Rubisco activase. This work highlights the need to better understand the correlative natural diversity between the Rubisco kinetics and CCM of diatoms and the underpinning mechanistic differences in catalytic chemistry among the Form I Rubisco superfamily.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.