ArticleJournal of nanobiotechnology2016
Contribution of actin filaments and microtubules to cell elongation and alignment depends on the grating depth of microgratings.
Article in Journal of nanobiotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Microtubules Disruption Alters the Cellular Structures and Mechanics Depending on Underlying Chemical Cues.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Evaluation of the Biocompatibility of Poly(benzimidazobenzophenanthroline)(BBL) Polymer Films with Living Cells.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- The Critical Role of the Shroom Family Proteins in Morphogenesis, Organogenesis and Disease.Phenomics (Cham, Switzerland) · 2024Review
- Effect of Anisotropic Structural Depth on Orientation and Differentiation Behavior of Skeletal Muscle Cells.ACS omega · 2023Article
- Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance.Materials today. Bio · 2023Article
- Nanotopography modulates cytoskeletal organization and dynamics during T cell activation.Molecular biology of the cell · 2022Article
- Article
- Effects of topographical guidance cues on osteoblast cell migration.Scientific reports · 2020Article
- Cellular and Subcellular Contact Guidance on Microfabricated Substrates.Frontiers in bioengineering and biotechnology · 2020Review
- Focal adhesion clustering drives endothelial cell morphology on patterned surfaces.Journal of the Royal Society, Interface · 2019Article
- The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance.Molecular biology of the cell · 2019Article
- Macrophage phenotype bioengineered by magnetic, genetic, or pharmacologic interference.Immunologic research · 2019Review
- Photo-patterned oxygen sensing films based on Pt porphyrin for controlling cell growth and studying metabolism.RSC advances · 2019Article
- Article
- Nanoengineered, cell-derived extracellular matrix influences ECM-related gene expression of mesenchymal stem cells.Biomaterials research · 2018Article
- Effect of Fibronectin-Coated Micro-Grooved Titanium Surface on Alignment, Adhesion, and Proliferation of Human Gingival Fibroblasts.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has been reported that both chemical and physical surface patterns influence cellular behaviors, such as cell alignment and elongation. However, it still remains unclear how actin filament and microtubules (MTs) differentially respond to these patterns.
resultsWe examined the effects of chemical and physical patterns on cell elongation and alignment by observing actin filament and MTs of retinal pigment epithelium-1(RPE-1) cells, which were cultured on either fibronectin (FN)-line pattern (line width and spacing: 1 μm) or FN-coated 1 μm gratings with two different depths (0.35 or 1 μm). On the surface with either FN-line pattern or micrograting structure, the cell aspect ratios were at least two times higher than those on the surface with no pattern. Cell elongation on the gratings depended on the depth of the gratings. Cell elongation and alignment on both FN-line pattern and 1 μm gratings with 0.35 μm depth were perturbed either by inhibition of actin polymerization or MT depletion, while cell elongation and alignment on 1 μm gratings with 1 μm depth were perturbed only by MT depletion.
conclusionsOur results suggest that the contribution of actin filaments and MTs to the elongation and alignment of epithelial cells on microgratings depends on the groove depth of these gratings.
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