ArticleThe Journal of biological chemistry2016
Quantitative Correlation between Infectivity and Gp120 Density on HIV-1 Virions Revealed by Optical Trapping Virometry.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- HIV-1 envelope glycoprotein modulates CXCR4 clustering and dynamics on the T cell membrane.eLife · 2026Article
- Virus-like antigen display delivers a stand-alone danger signal through the BCR that circumvents tolerance.bioRxiv : the preprint server for biology · 2026Article
- Quantifying Impact of HIV Receptor Surface Density Reveals Differences in Fusion Dynamics of HIV Strains.Viruses · 2025Article
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- Molecular ingredients of an immunogen for long-lasting IgG.Frontiers in immunology · 2025Review
- An Integrated Signaling Threshold Initiates IgG Response toward Virus-like Immunogens.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Molecular basis for potent B cell responses to antigen displayed on particles of viral size.Nature immunology · 2023Article
- Site-Specific and Stable Conjugation of the SARS-CoV-2 Receptor-Binding Domain to Liposomes in the Absence of Any Other Adjuvants Elicits Potent Neutralizing Antibodies in BALB/c Mice.Bioconjugate chemistry · 2021Article
- Neutralizing antibody responses to SARS-CoV-2 in symptomatic COVID-19 is persistent and critical for survival.Nature communications · 2021Article
- Measuring immunity to SARS-CoV-2 infection: comparing assays and animal models.Nature reviews. Immunology · 2020Review
- Probability of Immobilization on Host Cell Surface Regulates Viral Infectivity.Physical review letters · 2020Article
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- Synthetic Liposomal Mimics of Biological Viruses for the Study of Immune Responses to Infection and Vaccination.Bioconjugate chemistry · 2020Article
- Flow Cytometry Analysis of HIV-1 Env Conformations at the Surface of Infected Cells and Virions: Role of Nef, CD4, and SERINC5.Journal of virology · 2020Article
- A Coreceptor-Mimetic Peptide Enhances the Potency of V3-Glycan Antibodies.Journal of virology · 2019Article
- Back-focal-plane interferometric detection of nanoparticles in spatially confined microfluidic channels.The Review of scientific instruments · 2019Article
- Optimized protocol for detection of native, full-length HIV-1 envelope on the surface of transfected cells.Health science reports · 2018Article
- eCD4-Ig Variants That More Potently Neutralize HIV-1.Journal of virology · 2018Article
- Quantitation and Stability of Protein Conjugation on Liposomes for Controlled Density of Surface Epitopes.Bioconjugate chemistry · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The envelope glycoprotein (Env) gp120/gp41 is required for HIV-1 infection of host cells. Although in general it has been perceived that more Env gives rise to higher infectivity, the precise quantitative dependence of HIV-1 virion infectivity on Env density has remained unknown. Here we have developed a method to examine this dependence. This method involves 1) production of a set of single-cycle HIV-1 virions with varied density of Env on their surface, 2) site-specific labeling of Env-specific antibody Fab with a fluorophore at high efficiency, and 3) optical trapping virometry to measure the number of gp120 molecules on individual HIV-1 virions. The resulting gp120 density per virion is then correlated with the infectivity of the virions measured in cell culture. In the presence of DEAE-dextran, the polycation known to enhance HIV-1 infectivity in cell culture, virion infectivity follows gp120 density as a sigmoidal dependence and reaches an apparent plateau. This quantitative dependence can be described by a Hill equation, with a Hill coefficient of 2.4 ± 0.6. In contrast, in the absence of DEAE-dextran, virion infectivity increases monotonically with gp120 density and no saturation is observed under the experimental conditions. These results provide the first quantitative evidence that Env trimers cooperate on the virion surface to mediate productive infection by HIV-1. Moreover, as a result of the low number of Env trimers on individual virions, the number of additional Env trimers per virion that is required for the optimal infectivity will depend on the inclusion of facilitating agents during infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.