ArticleThe ISME journal2016
Identification of syntrophic acetate-oxidizing bacteria in anaerobic digesters by combined protein-based stable isotope probing and metagenomics.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Reduced methane emissions in transgenic rice genotypes are associated with altered rhizosphere microbial hydrogen cycling.Nature communications · 2026Article
- Biowaste-grown live microbial feed additive sustainably and significantly cut enteric methane emissions in Indian livestock.Scientific reports · 2026Article
- Aerith: Visualization and Annotation of Isotopic Enrichment Patterns of Peptides and Metabolites with Stable Isotope Labeling from Proteomics and Metabolomics.Analytical chemistry · 2025Article
- Activity-targeted metaproteomics uncovers rare syntrophic bacteria central to anaerobic community metabolism.Nature microbiology · 2025Article
- Identifying rare and active species that drive carbon turnover in complex microbiotas.Nature microbiology · 2025Article
- Mid-day photomixotrophy byApplied and environmental microbiology · 2025Article
- Identification of protein-degraders in an anaerobic digester by protein stable isotope probing and metagenomics.Applied microbiology and biotechnology · 2025Article
- Sustainable Food Waste Management in Anaerobic Digesters: Prediction of the Organic Load Impact by Metagenome-Scale Metabolic Modeling.Environmental science & technology · 2025Article
- Advances in actinobacteria-based bioremediation: mechanistic insights, genetic regulation, and emerging technologies.Biodegradation · 2025Review
- Core cooperative metabolism in low-complexity CO2-fixing anaerobic microbiota.The ISME journal · 2025Article
- Evaluation of microbial diversity in the formation water of the producer and marginal wells in bokaro coal field.Scientific reports · 2024Article
- Metabolism of novel potential syntrophic acetate-oxidizing bacteria in thermophilic methanogenic chemostats.Applied and environmental microbiology · 2024Article
- Treatment of sugarcane vinasse in AnMBR and UASB: process performance and microbial community comparison.Frontiers in bioengineering and biotechnology · 2024Article
- Diverse electron carriers drive syntrophic interactions in an enriched anaerobic acetate-oxidizing consortium.The ISME journal · 2023Article
- Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics.Biotechnology for biofuels and bioproducts · 2023Article
- Evaluation of Nanaerobic Digestion as a Mechanism to Explain Surplus Methane Production in Animal Rumina and Engineered Digesters.Environmental science & technology · 2023Article
- Biomethane production and microbial strategies corresponding to high organic loading treatment for molasses wastewater in an upflow anaerobic filter reactor.Bioprocess and biosystems engineering · 2023Article
- Syntrophic Propionate Oxidation: One of the Rate-Limiting Steps of Organic Matter Decomposition in Anoxic Environments.Applied and environmental microbiology · 2023Review
- Effects of phenyl acids on different degradation phases during thermophilic anaerobic digestion.Frontiers in microbiology · 2023Article
- Adaptation of a microbial community to demand-oriented biological methanation.Biotechnology for biofuels and bioproducts · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhibition of anaerobic digestion through accumulation of volatile fatty acids occasionally occurs as the result of unbalanced growth between acidogenic bacteria and methanogens. A fast recovery is a prerequisite for establishing an economical production of biogas. However, very little is known about the microorganisms facilitating this recovery. In this study, we investigated the organisms involved by a novel approach of mapping protein-stable isotope probing (protein-SIP) onto a binned metagenome. Under simulation of acetate accumulation conditions, formations of (13)C-labeled CO2 and CH4 were detected immediately following incubation with [U-(13)C]acetate, indicating high turnover rate of acetate. The identified (13)C-labeled peptides were mapped onto a binned metagenome for improved identification of the organisms involved. The results revealed that Methanosarcina and Methanoculleus were actively involved in acetate turnover, as were five subspecies of Clostridia. The acetate-consuming organisms affiliating with Clostridia all contained the FTFHS gene for formyltetrahydrofolate synthetase, a key enzyme for reductive acetogenesis, indicating that these organisms are possible syntrophic acetate-oxidizing (SAO) bacteria that can facilitate acetate consumption via SAO, coupled with hydrogenotrophic methanogenesis (SAO-HM). This study represents the first study applying protein-SIP for analysis of complex biogas samples, a promising method for identifying key microorganisms utilizing specific pathways.
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