ArticleToxins2016
Deciphering the Anti-Aflatoxinogenic Properties of Eugenol Using a Large-Scale q-PCR Approach.
Article in Toxins, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- A multi-barrier defence strategy using plant-derived antifungals against mycotoxigenic fungi in livestock feed.Archives of microbiology · 2026Review
- Review
- Inhibition Mechanism ofFoods (Basel, Switzerland) · 2025Article
- Inhibitive effect of Urginea epigea methanolic extract and silver/zinc oxide nanoparticles on Aspergillus and aflatoxin production.PloS one · 2025Article
- Identification and evaluation of suitable reference genes for RT-qPCR analyses in Trichoderma atroviride under varying light conditions.Fungal biology and biotechnology · 2023Article
- Role of Polyphenols from the Aqueous Extract ofMolecules (Basel, Switzerland) · 2023Article
- A putative terpene cyclase gene (Frontiers in microbiology · 2023Article
- Inhibition of Aflatoxin B1 Synthesis inMolecules (Basel, Switzerland) · 2022Article
- Phenolic Compounds Recovery from Blood Orange Peels Using a Novel Green Infrared TechnologyMolecules (Basel, Switzerland) · 2022Article
- Inhibition ofToxins · 2022Article
- A methanolic extract of Zanthoxylum bungeanum modulates secondary metabolism regulator genes in Aspergillus flavus and shuts down aflatoxin production.Scientific reports · 2022Article
- The Solvent Dimethyl Sulfoxide Affects Physiology, Transcriptome and Secondary Metabolism ofJournal of fungi (Basel, Switzerland) · 2021Article
- Review
- Article
- The AFLATOXInternational journal of molecular sciences · 2021Article
- The Potential of Plant-Based Bioactive Compounds on Inhibition of Aflatoxin B1 Biosynthesis and Down-regulation ofAntibiotics (Basel, Switzerland) · 2020Article
- Review
- Sabotage at the Powerhouse? Unraveling the Molecular Target of 2-Isopropylbenzaldehyde Thiosemicarbazone, a Specific Inhibitor of Aflatoxin Biosynthesis and Sclerotia Development inMolecules (Basel, Switzerland) · 2019Article
- Article
- Ethanol Inhibits Aflatoxin BFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Produced by several species of Aspergillus, Aflatoxin B₁ (AFB₁) is a carcinogenic mycotoxin contaminating many crops worldwide. The utilization of fungicides is currently one of the most common methods; nevertheless, their use is not environmentally or economically sound. Thus, the use of natural compounds able to block aflatoxinogenesis could represent an alternative strategy to limit food and feed contamination. For instance, eugenol, a 4-allyl-2-methoxyphenol present in many essential oils, has been identified as an anti-aflatoxin molecule. However, its precise mechanism of action has yet to be clarified. The production of AFB₁ is associated with the expression of a 70 kB cluster, and not less than 21 enzymatic reactions are necessary for its production. Based on former empirical data, a molecular tool composed of 60 genes targeting 27 genes of aflatoxin B₁ cluster and 33 genes encoding the main regulatory factors potentially involved in its production, was developed. We showed that AFB₁ inhibition in Aspergillus flavus following eugenol addition at 0.5 mM in a Malt Extract Agar (MEA) medium resulted in a complete inhibition of the expression of all but one gene of the AFB₁ biosynthesis cluster. This transcriptomic effect followed a down-regulation of the complex composed by the two internal regulatory factors, AflR and AflS. This phenomenon was also influenced by an over-expression of veA and mtfA, two genes that are directly linked to AFB₁ cluster regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.