ArticleACS chemical biology2016
Sequestration-Mediated Downregulation of de Novo Purine Biosynthesis by AMPK.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- The oncogenic control of nucleotide synthesis.Oncogenesis · 2026Review
- Cellular view of metabolism: metabolic biomolecular condensates.The New phytologist · 2025Review
- Purinosomes and Purine Metabolism in Mammalian Neural Development: A Review.Acta histochemica et cytochemica · 2024Review
- Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assembly.The Journal of cell biology · 2024Article
- Purinosomes involved in the regulation of tumor metabolism: current progress and potential application targets.Frontiers in oncology · 2024Review
- A journey into the regulatory secrets of theFrontiers in pharmacology · 2024Review
- Metabolic channeling: predictions, deductions, and evidence.Molecular cell · 2021Review
- Review
- Human de novo purine biosynthesis.Critical reviews in biochemistry and molecular biology · 2021Review
- ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.Molecular cell · 2020Article
- Regulation of reaction fluxes via enzyme sequestration and co-clustering.Journal of the Royal Society, Interface · 2019Article
- Review
- Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome.Methods in enzymology · 2019Article
- The run-on oligomer filament enzyme mechanism of SgrAI: Part 2. Kinetic modeling of the full DNA cleavage pathway.The Journal of biological chemistry · 2018Article
- The run-on oligomer filament enzyme mechanism of SgrAI: Part 1. Assembly kinetics of the run-on oligomer filament.The Journal of biological chemistry · 2018Article
- Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase in neurons.Journal of neurochemistry · 2018Article
- Spatial alterations of De Novo purine biosynthetic enzymes by Akt-independent PDK1 signaling pathways.PloS one · 2018Article
- Detecting Purinosome Metabolon Formation with Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Spatial Organization of Metabolic Enzyme Complexes in Cells.Biochemistry · 2017Review
- A New View into the Regulation of Purine Metabolism: The Purinosome.Trends in biochemical sciences · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Dynamic partitioning of de novo purine biosynthetic enzymes into multienzyme compartments, purinosomes, has been associated with increased flux of de novo purine biosynthesis in human cells. However, we do not know of a mechanism by which de novo purine biosynthesis would be downregulated in cells. We have investigated the functional role of AMP-activated protein kinase (AMPK) in the regulation of de novo purine biosynthesis because of its regulatory action on lipid and carbohydrate biosynthetic pathways. Using pharmacological AMPK activators, we have monitored subcellular localizations of six pathway enzymes tagged with green fluorescent proteins under time-lapse fluorescence single-cell microscopy. We revealed that only one out of six pathway enzymes, formylglycinamidine ribonucleotide synthase (FGAMS), formed spatially distinct cytoplasmic granules after treatment with AMPK activators, indicating the formation of single-enzyme self-assemblies. In addition, subsequent biophysical studies using fluorescence recovery after photobleaching showed that the diffusion kinetics of FGAMS were slower when it localized inside the self-assemblies than within the purinosomes. Importantly, high-performance liquid chromatographic studies revealed that the formation of AMPK-promoted FGAMS self-assembly caused the reduction of purine metabolites in HeLa cells, indicating the downregulation of de novo purine biosynthesis. Collectively, we demonstrate here that the spatial sequestration of FGAMS by AMPK is a mechanism by which de novo purine biosynthesis is downregulated in human cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.