ArticlePloS one2016
DNA Barcoding of the Endangered Aquilaria (Thymelaeaceae) and Its Application in Species Authentication of Agarwood Products Traded in the Market.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 91 citations in OpenAlex.
- Advancing plant DNA barcoding: integrating chloroplast genome sequencing, cryptic diversity discovery and machine learning.Molecular biology reports · 2026Review
- DNA Barcoding Provides Taxonomic Clues for Identifying Five EndangeredPlants (Basel, Switzerland) · 2025Article
- Use of Multi-Locus Metabarcoding to Inform an Australian Government Biosecurity Response on the Origins of Suspected Illegal Plant Products.International journal of molecular sciences · 2025Article
- Conservation Strategies for Aquilaria sinensis: Insights from DNA Barcoding and ISSR Markers.Plant foods for human nutrition (Dordrecht, Netherlands) · 2024Article
- Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome.Frontiers in plant science · 2024Article
- Identification of the Plant Family Caryophyllaceae in Korea Using DNA Barcoding.Plants (Basel, Switzerland) · 2023Article
- Highly divergent regions in the complete plastome sequences of3 Biotech · 2023Article
- Comparison of DNA extraction methods on CITES-listed timber species and application in species authentication of commercial products using DNA barcoding.Scientific reports · 2023Article
- Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review.AoB PLANTS · 2022Review
- Comparison of phenolic compounds contained in Aquilaria leaves of different species.Journal of natural medicines · 2022Article
- Biotechnological Advances in Pharmacognosy and In Vitro Manipulation ofPlants (Basel, Switzerland) · 2022Review
- Genetic relationship and source species identification of 58 Qi-Nan germplasms of Aquilaria species in China that easily form agarwood.PloS one · 2022Article
- A Concise Review ofPlants (Basel, Switzerland) · 2021Review
- Molecular identification ofMitochondrial DNA. Part B, Resources · 2021Article
- Article
- Comparison of eight complete chloroplast genomes of the endangered Aquilaria tree species (Thymelaeaceae) and their phylogenetic relationships.Scientific reports · 2020Article
- DNA barcodes for the identification of Stephania (Menispermaceae) species.Molecular biology reports · 2020Article
- Cross-amplification of microsatellite markers across agarwood-producing species of the Aquilarieae tribe (Thymelaeaceae).3 Biotech · 2020Article
- Characterization of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2020Article
- DNA barcoding supports existence of morphospecies complex in endemic bamboo genusJournal of genetics · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification of Aquilaria species from their resinous non-wood product, the agarwood, is challenging as conventional techniques alone are unable to ascertain the species origin. Aquilaria is a highly protected species due to the excessive exploitation of its precious agarwood. Here, we applied the DNA barcoding technique to generate barcode sequences for Aquilaria species and later applied the barcodes to identify the source species of agarwood found in the market. We developed a reference DNA barcode library using eight candidate barcode loci (matK, rbcL, rpoB, rpoC1, psbA-trnH, trnL-trnF, ITS, and ITS2) amplified from 24 leaf accessions of seven Aquilaria species obtained from living trees. Our results indicated that all single barcodes can be easily amplified and sequenced with the selected primers. The combination of trnL-trnF+ITS and trnL-trnF+ITS2 yielded the greatest species resolution using the least number of loci combination, while matK+trnL-trnF+ITS showed potential in detecting the geographical origins of Aquilaria species. We propose trnL-trnF+ITS2 as the best candidate barcode for Aquilaria as ITS2 has a shorter sequence length compared to ITS, which eases PCR amplification especially when using degraded DNA samples such as those extracted from processed agarwood products. A blind test conducted on eight agarwood samples in different forms using the proposed barcode combination proved successful in their identification up to the species level. Such potential of DNA barcoding in identifying the source species of agarwood will contribute to the international timber trade control, by providing an effective method for species identification and product authentication.
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