Evidence map›Paper›PMID 27127340›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2016

Development of a double-monoclonal antibody sandwich ELISA: Tool for chicken interferon-γ detection ex vivo.

Hua Dai, Zheng-Zhong Xu, Meiling Wang, Jun-Hua Chen, Xiang Chen, Zhi-Ming Pan, Xin-An Jiao

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hua DaiJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Zheng-Zhong XuJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Meiling WangJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Jun-Hua ChenJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Xiang ChenJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Zhi-Ming PanJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Xin-An JiaoJiangsu Key Lab of Zoonosis, Yangzhou University, China (Dai, Xu, Chen, Chen X, Pan, Jiao); College of Medicine, Yangzhou University, China (Wang).
Yangzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present work was to develop reagents to set up a chicken interferon-γ (ChIFN-γ) assay. Four monoclonal antibodies (mAbs) specific for ChIFN-γ were generated to establish sandwich ELISA based on 2 different mAbs. To improve the detection sensitivity of ChIFN-γ, a double-monoclonal antibody sandwich ELISA was developed using mAb 3E5 as capture antibody and biotinylated mAb 3E3 as a detection reagent. The results revealed that this ELISA has high sensitivity, allowing for the detection of 125 to 500 pg/mL of recombinant ChIFN-γ, and also has an excellent capacity for detecting native ChIFN-γ. This ELISA was then used to detect ChIFN-γ level in chickens immunized with a Newcastle disease virus (NDV) vaccine, the immunized chicken splenocytes were stimulated by NDV F protein as recall antigen. From our results, it appears that the sensitivity range of this sandwich ELISA test is adequate to measure the ex vivo release of ChIFN-γ.

Indexed as

AnimalsAntibodies, MonoclonalChickensEnzyme-Linked Immunosorbent AssayGene Expression RegulationInterferon-gammaMiceMice, Inbred BALB CNewcastle DiseasePlasmidsRecombinant ProteinsSensitivity and SpecificityViral VaccinesAntibodies, MonoclonalInterferon-gammaRecombinant ProteinsViral Vaccines

Identifiers

PMID27127340
PMCPMC4836039
OpenAlexW2395725617

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.