ArticleScientific reports2016
In vivo assembly of DNA-fragments in the moss, Physcomitrella patens.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- The Importance of thePlants (Basel, Switzerland) · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Engineering the mossFrontiers in plant science · 2025Article
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- A designer synthetic chromosome fragment functions in moss.Nature plants · 2024Article
- In Vivo DNA Assembly Using the PEDA Method.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Heterologous Production of Artemisinin inBio-protocol · 2023Article
- Overexpression of Physcomitrium patens cell cycle regulators leads to larger gametophytes.Scientific reports · 2023Article
- From Plant to Yeast-Advances in Biosynthesis of Artemisinin.Molecules (Basel, Switzerland) · 2022Review
- Plant cell cultures as heterologous bio-factories for secondary metabolite production.Plant communications · 2021Review
- Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts.Bio-protocol · 2021Article
- Traps and Pitfalls-Unspecific Reactions in Metabolic Engineering of Sesquiterpenoid Pathways.Molecules (Basel, Switzerland) · 2020Review
- Connecting moss lipid droplets to patchoulol biosynthesis.PloS one · 2020Article
- Article
- Physcomitrella patens, a versatile synthetic biology chassis.Plant cell reports · 2018Review
- An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing.Scientific reports · 2017Article
- A Review of Biotechnological Artemisinin Production in Plants.Frontiers in plant science · 2017Review
- Stable Production of the Antimalarial Drug Artemisinin in the MossFrontiers in bioengineering and biotechnology · 2017Article
- The decision to germinate is regulated by divergent molecular networks in spores and seeds.The New phytologist · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complete replacement of eukaryotic chromosomes with heterologous DNA. The moss Physcomitrella patens, a non-vascular and spore producing land plant (Bryophyte), has a well-established capacity for homologous recombination. Here, we demonstrate the in vivo assembly of multiple DNA fragments in P. patens with three examples of effective genome editing: we (i) efficiently deleted a genomic locus for diterpenoid metabolism yielding a biosynthetic knockout, (ii) introduced a salt inducible promoter, and (iii) re-routed endogenous metabolism into the formation of amorphadiene, a precursor of high-value therapeutics. These proof-of-principle experiments pave the way for more complex and increasingly flexible approaches for large-scale metabolic engineering in plant biotechnology.
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