ArticleJournal of the American Society for Mass Spectrometry2016
Reducing Spatial Heterogeneity of MALDI Samples with Marangoni Flows During Sample Preparation.
Article in Journal of the American Society for Mass Spectrometry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- A Fast-Pass, Desorption Electrospray Ionization Mass Spectrometry Strategy for Untargeted Metabolic Phenotyping.Journal of the American Society for Mass Spectrometry · 2025Article
- Microarray-Based Methodology for Lipid Profiling, Enzymatic Activity, And Binding Assays in Printed Lipid Raft Membranes from Astrocytes and Neurons.Analytical chemistry · 2025Article
- Recent Applications of Artificial Intelligence and Related Technical Challenges in MALDI MS and MALDI-MSI: A Mini Review.Mass spectrometry (Tokyo, Japan) · 2025Review
- Control of the Drying Patterns for Complex Colloidal Solutions and Their Applications.Nanomaterials (Basel, Switzerland) · 2022Review
- Microbowls with Controlled Concavity for Accurate Microscale Mass Spectrometry.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- Mass Spectrometry Imaging of Biological Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry.Journal of visualized experiments : JoVE · 2018Article
- Mass Spectrometry Imaging: A Review of Emerging Advancements and Future Insights.Analytical chemistry · 2018Review
- Critical factors determining the quantification capability of matrix-assisted laser desorption/ionization- time-of-flight mass spectrometry.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Review
- Preparation of Homogeneous MALDI Samples for Quantitative Applications.Journal of visualized experiments : JoVE · 2016Article
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8 authors.
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Abstract
This work demonstrates a method to prepare homogeneous distributions of analytes to improve data reproducibility in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). Natural-air drying processes normally result in unwanted heterogeneous spatial distributions of analytes in MALDI crystals and make quantitative analysis difficult. This study demonstrates that inducing Marangoni flows within drying droplets can significantly reduce the heterogeneity problem. The Marangoni flows are accelerated by changing substrate temperatures to create temperature gradients across droplets. Such hydrodynamic flows are analyzed semi-empirically. Using imaging mass spectrometry, changes of heterogeneity of molecules with the change of substrate temperature during drying processes are demonstrated. The observed heterogeneities of the biomolecules reduce as predicted Marangoni velocities increase. In comparison to conventional methods, drying droplets on a 5 °C substrate while keeping the surroundings at ambient conditions typically reduces the heterogeneity of biomolecular ions by 65%-80%. The observation suggests that decreasing substrate temperature during droplet drying processes is a simple and effective means to reduce analyte heterogeneity for quantitative applications. Graphical Abstract ᅟ.
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