ArticleScientific reports2016
Characterization of key triacylglycerol biosynthesis processes in rhodococci.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Nitrogen limitation causes a seismic shift in redox state and phosphorylation of proteins implicated in carbon flux and lipidome remodeling in Rhodotorula toruloides.Biotechnology for biofuels and bioproducts · 2025Article
- Increased triacylglycerol production in Rhodococcus opacus by overexpressing transcriptional regulators.Biotechnology for biofuels and bioproducts · 2024Article
- Harnessing redox proteomics to study metabolic regulation and stress response in lignin-fed Rhodococci.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering ofHeliyon · 2023Article
- Genetic analysis of acyl-CoA carboxylases involved in lipid accumulation in Rhodococcus jostii RHA1.Applied microbiology and biotechnology · 2023Article
- High wax ester and triacylglycerol biosynthesis potential in coastal sediments of Antarctic and Subantarctic environments.PloS one · 2023Article
- Mineral-hydrogel composites for mitigating harmful algal bloom and supplying phosphorous for photo-biorefineries.The Science of the total environment · 2022Article
- Exposure to Trypanosoma parasites induces changes in the microbiome of the Chagas disease vector Rhodnius prolixus.Microbiome · 2022Article
- Recent Biotechnology Advances in Bio-Conversion of Lignin to Lipids by Bacterial Cultures.Frontiers in chemistry · 2022Review
- Review
- Dual-function oleaginous biocatalysts for non-sterile cultivation and solvent-free biolipid bioextraction to reduce biolipid-based biofuel production costs.The Science of the total environment · 2021Article
- Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440.Biotechnology for biofuels · 2021Article
- Steryl Ester Formation and Accumulation in Steroid-Degrading Bacteria.Applied and environmental microbiology · 2020Article
- Insights into the Metabolism of OleaginousApplied and environmental microbiology · 2019Review
- Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation ofBiotechnology for biofuels · 2019Article
- Article
- Dynamic decoupling of biomass and wax ester biosynthesis inMetabolic engineering communications · 2018Article
- Stepwise metabolic engineering ofBiotechnology for biofuels · 2018Article
- A Fatty Acyl Coenzyme A Reductase Promotes Wax Ester Accumulation in Rhodococcus jostii RHA1.Applied and environmental microbiology · 2017Article
- Engineering levoglucosan metabolic pathway in Rhodococcus jostii RHA1 for lipid production.Journal of industrial microbiology & biotechnology · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oleaginous microorganisms have considerable potential for biofuel and commodity chemical production. Under nitrogen-limitation, Rhodococcus jostii RHA1 grown on benzoate, an analog of lignin depolymerization products, accumulated triacylglycerols (TAGs) to 55% of its dry weight during transition to stationary phase, with the predominant fatty acids being C16:0 and C17:0. Transcriptomic analyses of RHA1 grown under conditions of N-limitation and N-excess revealed 1,826 dysregulated genes. Genes whose transcripts were more abundant under N-limitation included those involved in ammonium assimilation, benzoate catabolism, fatty acid biosynthesis and the methylmalonyl-CoA pathway. Of the 16 atf genes potentially encoding diacylglycerol O-acyltransferases, atf8 transcripts were the most abundant during N-limitation (~50-fold more abundant than during N-excess). Consistent with Atf8 being a physiological determinant of TAG accumulation, a Δatf8 mutant accumulated 70% less TAG than wild-type RHA1 while atf8 overexpression increased TAG accumulation 20%. Genes encoding type-2 phosphatidic acid phosphatases were not significantly expressed. By contrast, three genes potentially encoding phosphatases of the haloacid dehalogenase superfamily and that cluster with, or are fused with other Kennedy pathway genes were dysregulated. Overall, these findings advance our understanding of TAG metabolism in mycolic acid-containing bacteria and provide a framework to engineer strains for increased TAG production.
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