ArticleMicrobial cell factories2016
The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Overcoming cellular secretion bottlenecks: advanced secretion engineering and molecular tailoring for next-generation microbial α-amylases with enhanced industrial performance.World journal of microbiology & biotechnology · 2026Review
- Article
- Optimization of response surface methodology for the extraction of isoliquiritigenin fromFrontiers in pharmacology · 2025Article
- Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass.Microbial cell factories · 2024Article
- Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila.Microbial cell factories · 2023Article
- A novel sucrose-inducible expression system and its application for production of biomass-degrading enzymes in Aspergillus niger.Biotechnology for biofuels and bioproducts · 2023Article
- Construction of a novel filamentous fungal protein expression system based on redesigning of regulatory elements.Applied microbiology and biotechnology · 2022Article
- CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars.Biotechnology for biofuels · 2021Article
- Biosynthesis and industrial applications of α-amylase: a review.Archives of microbiology · 2021Review
- Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium.Microbial cell factories · 2020Article
- Improved Homologous Expression of the Acidic Lipase fromJournal of microbiology and biotechnology · 2020Article
- Novel amylase-producing fungus hydrolyzing wheat and brewing residues, Aspergillus carbonarius, discovered in tropical forest remnant.Folia microbiologica · 2020Article
- The transcription factor Zt107320 affects the dimorphic switch, growth and virulence of the fungal wheat pathogen Zymoseptoria tritici.Molecular plant pathology · 2020Article
- Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.Microbial cell factories · 2019Review
- Editorial: Advances in the Regulation and Production of Fungal Enzymes by Transcriptomics, Proteomics and Recombinant Strains Design.Frontiers in bioengineering and biotechnology · 2019Article
- Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum.Applied and environmental microbiology · 2018Article
- The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression inBiotechnology for biofuels · 2018Article
- Deletion of TpKu70 facilitates gene targeting in Talaromyces pinophilus and identification of TpAmyR involvement in amylase production.World journal of microbiology & biotechnology · 2017Article
- Article
- Biotechnological Processes in Microbial Amylase Production.BioMed research international · 2017Review
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7 authors.
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Abstract
backgroundThe filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins in these recombinant strains still remain, such as alpha-amylase and alpha-glucosidase; lead to a low-purity of fermentation products. The aim was to construct an A. niger host strain with a low background of protein secretion.
resultsThe transcription factor amyR was deleted in A. niger CICC2462, and the results from enzyme activity assays and SDS-PAGE analysis showed that the glucoamylase and amylase activities of the ∆amyR strains were significantly lower than those of the wild-type strain. High-throughput RNA-sequencing and shotgun LC-MS/MS proteomic technology analysis demonstrated that the expression of amylolytic enzymes was decreased at both the transcriptional and translational levels in the ∆amyR strain. Interestingly, the ∆amyR strain growth rate better than the wild-type strain.
conclusionsOur findings clearly indicated that the ∆amyR strain of A. niger CICC2462 can be used as a host strain with a low background of protein secretion.
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