Evidence map›Paper›PMID 27125356›Full record

ArticleModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc2016

Definition of MYC genetic heteroclonality in diffuse large B-cell lymphoma with 8q24 rearrangement and its impact on protein expression.

Alexandra Valera, Samantha Epistolio, Lluis Colomo, Alice Riva, Olga Balagué, Ivan Dlouhy, Alexandar Tzankov, Marco Bühler, Eugenia Haralambieva, Elias Campo and 3 more

Open access · bronzeAbstract readMulticenter Study
PubMed Publisher
In one paragraph

Article in Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. IgA-producing lymphoplasmacytic lymphoma carrying the chromosomal abnormality t(8;14).Journal of clinical and experimental hematopathology : JCEH · 2019
    Article
  5. CD5-Positive Primary Cutaneous Diffuse Large B-Cell Lymphoma-Leg Type.Case reports in dermatological medicine · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Alexandra ValeraDepartment of Molecular Pathology, Institute of Pathology, Locarno, Switzerland.
Samantha EpistolioDepartment of Molecular Pathology, Institute of Pathology, Locarno, Switzerland.
Lluis ColomoLaboratory of Pathology, Hospital del Mar, Universitat Pompeu Fabra, Barcelona, Spain.
Alice RivaDepartment of Molecular Pathology, Institute of Pathology, Locarno, Switzerland.
Olga BalaguéHematopathology Section, Laboratory of Pathology, Hospital Clínic, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.
Ivan DlouhyHematopathology Section, Laboratory of Pathology, Hospital Clínic, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.
Alexandar TzankovInstitute of Pathology, University Hospital, Basel, Switzerland.ORCID 0000-0002-1100-3819
Marco BühlerInstitute for Surgical Pathology, University Hospital and University of Zurich, Zurich, Switzerland.
Eugenia HaralambievaInstitute for Surgical Pathology, University Hospital and University of Zurich, Zurich, Switzerland.
Elias CampoHematopathology Section, Laboratory of Pathology, Hospital Clínic, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.ORCID 0000-0001-9850-9793
Davide SoldiniInstitute for Surgical Pathology, University Hospital and University of Zurich, Zurich, Switzerland.
Luca MazzucchelliDepartment of Molecular Pathology, Institute of Pathology, Locarno, Switzerland.
Vittoria MartinDepartment of Molecular Pathology, Institute of Pathology, Locarno, Switzerland.
General Electric (Switzerland) · CHConsorci Institut D'Investigacions Biomediques August Pi I Sunyer · ESUniversity of Zurich · CHUniversitat Pompeu Fabra · ESUniversity Hospital of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MYC rearrangement can be detected in a subgroup of diffuse large B-cell lymphoma characterized by unfavorable prognosis. In contrast to Burkitt lymphoma, the correlation between MYC rearrangement and MYC protein expression in diffuse large B-cell lymphoma is less clear, as approximately one-third of rearranged cases show negative or low expression by immunohistochemistry. To better understand whether specific characteristics of the MYC rearrangement may influence its protein expression, we investigated 43 de novo diffuse large B-cell lymphoma positive for 8q24 rearrangement by FISH, using 14 Burkitt lymphoma for comparison. Different cell populations (clones), breakpoints (classical vs non-classical FISH patterns), partner genes (IGH vs non-IGH) and immunostaining were detected and analyzed using computerized image systems. In a subgroup of diffuse large B-cell lymphoma, we observed different clones within the same tumor distinguishing the founder clone with MYC rearrangement alone from other subclones, carrying MYC rearrangement coupled with loss/extra copies of derivatives/normal alleles. This picture, which we defined MYC genetic heteroclonality, was found in 42% of cases and correlated to negative MYC expression (P=0.026). Non-classical FISH breakpoints were detected in 16% of diffuse large B-cell lymphoma without affecting expression (P=0.040). Non-IGH gene was the preferential partner of rearrangement in those diffuse large B-cell lymphoma showing MYC heteroclonality (P=0.016) and/or non-classical FISH breakpoints (P=0.058). MYC heteroclonality was not observed in Burkitt lymphoma and all cases had positive MYC expression. Non-classical FISH MYC breakpoint and non-IGH partner were found in 29 and 20% of Burkitt lymphoma, respectively. In conclusion, MYC genetic heteroclonality is a frequent event in diffuse large B-cell lymphoma and may have a relevant role in modulating MYC expression.

Indexed as

Chromosomes, Human, Pair 8Gene RearrangementAdultAgedAged, 80 and overBiomarkers, TumorFemaleGene Expression Regulation, NeoplasticGenes, Immunoglobulin Heavy ChainGenetic Predisposition to DiseaseHumansImmunohistochemistryIn Situ Hybridization, FluorescenceLymphoma, Large B-Cell, DiffuseMaleMiddle AgedBiomarkers, TumorMYC protein, humanProto-Oncogene Proteins c-myc

Identifiers

PMID27125356
OpenAlexW2342950176

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.