ArticleCell cycle (Georgetown, Tex.)2017
Mesenchymal stromal cells having inactivated RB1 survive following low irradiation and accumulate damaged DNA: Hints for side effects following radiotherapy.
Article in Cell cycle (Georgetown, Tex.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 19 citations in OpenAlex.
- Competing risk nomogram for predicting cancer-specific survival in patients with primary bone diffuse large B-cell lymphoma: a SEER-based retrospective study.Frontiers in medicine · 2025Article
- The emerging role of IL-17A across different types of radiation-induced normal tissue injuries.Therapeutic advances in medical oncology · 2025Review
- Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress.Journal of personalized medicine · 2022Article
- Radiomics as a New Frontier of Imaging for Cancer Prognosis: A Narrative Review.Diagnostics (Basel, Switzerland) · 2021Review
- Radiomics in the Setting of Neoadjuvant Radiotherapy: A New Approach for Tailored Treatment.Cancers · 2021Review
- Radiation induces primary osteocyte senescence phenotype and affects osteoclastogenesisInternational journal of molecular medicine · 2021Article
- The Role of Mesenchymal Stem Cells in Radiation-Induced Lung Fibrosis.International journal of molecular sciences · 2019Review
- A rapid, safe, and quantitative in vitro assay for measurement of uracil-DNA glycosylase activity.Journal of molecular medicine (Berlin, Germany) · 2019Article
- Senescence Phenomena and Metabolic Alteration in Mesenchymal Stromal Cells from a Mouse Model of Rett Syndrome.International journal of molecular sciences · 2019Article
- Regulatory roles of miR-22/Redd1-mediated mitochondrial ROS and cellular autophagy in ionizing radiation-induced BMSC injury.Cell death & disease · 2019Article
- Stress and stem cells: adult Muse cells tolerate extensive genotoxic stimuli better than mesenchymal stromal cells.Oncotarget · 2018Article
- Bone marrow-derived mesenchymal stromal cells promote colorectal cancer cell death under low-dose irradiation.British journal of cancer · 2018Article
- Relevance of 3d culture systems to study osteosarcoma environment.Journal of experimental & clinical cancer research : CR · 2018Review
- Radiation Induces Apoptosis and Osteogenic Impairment through miR-22-Mediated Intracellular Oxidative Stress in Bone Marrow Mesenchymal Stem Cells.Stem cells international · 2018Article
- γH2AX, 53BP1 and Rad51 protein foci changes in mesenchymal stem cells during prolonged X-ray irradiation.Oncotarget · 2017Article
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9 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Following radiotherapy, bone sarcomas account for a significant percentage of recurring tumors. This risk is further increased in patients with hereditary retinoblastoma that undergo radiotherapy. We analyzed the effect of low and medium dose radiation on mesenchymal stromal cells (MSCs) with inactivated RB1 gene to gain insights on the molecular mechanisms that can induce second malignant neoplasm in cancer survivors. MSC cultures contain subpopulations of mesenchymal stem cells and committed progenitors that can differentiate into mesodermal derivatives: adipocytes, chondrocytes, and osteocytes. These stem cells and committed osteoblast precursors are the cell of origin in osteosarcoma, and RB1 gene mutations have a strong role in its pathogenesis. Following 40 and 2000 mGy X-ray exposure, MSCs with inactivated RB1 do not proliferate and accumulate high levels of unrepaired DNA as detected by persistence of gamma-H2AX foci. In samples with inactivated RB1 the radiation treatment did not increase apoptosis, necrosis or senescence versus untreated cells. Following radiation, CFU analysis showed a discrete number of cells with clonogenic capacity in cultures with silenced RB1. We extended our analysis to the other members of retinoblastoma gene family: RB2/P130 and P107. Also in the MSCs with silenced RB2/P130 and P107 we detected the presence of cells with unrepaired DNA following X-ray irradiation. Cells with unrepaired DNA may represent a reservoir of cells that may undergo neoplastic transformation. Our study suggests that, following radiotherapy, cancer patients with mutations of retinoblastoma genes may be under strict controls to evaluate onset of secondary neoplasms following radiotherapy.
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