ArticleProtein science : a publication of the Protein Society2016
Metal binding affinity and structural properties of calmodulin-like protein 14 from Arabidopsis thaliana.
Article in Protein science : a publication of the Protein Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 36 citations in OpenAlex.
- The calmodulin-like proteins, CML13 and CML14 function as myosin light chains for the class XI myosins in Arabidopsis.Plant & cell physiology · 2026Article
- TaCML49-B, a Calmodulin-like Protein, Interacts with TaIQD23 to Positively Regulate Salt Tolerance in Wheat.Plants (Basel, Switzerland) · 2025Article
- Characterization of the Calmodulin-Like Protein Family in Chara braunii and their Conserved Interaction with the Calmodulin-Binding Transcription Activator Family.Plant & cell physiology · 2024Article
- A chloroplast localized heavy metal-associated domain containing protein regulates grain calcium accumulation in rice.Nature communications · 2024Article
- Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins.Journal of experimental botany · 2024Article
- Conformational Plasticity of Centrin 1 fromBiomolecules · 2022Article
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- The interplay of self-assembly and target binding in centrin 1 from Toxoplasma gondii.The Biochemical journal · 2021Article
- Cystathionine β-synthase is involved in cysteine biosynthesis and HScientific reports · 2020Article
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- In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.Plant physiology · 2020Review
- Functional Characterization and Structure-Guided Mutational Analysis of the Transsulfuration Enzyme Cystathionine γ-Lyase fromInternational journal of molecular sciences · 2018Article
- Towards Understanding Plant Calcium Signaling through Calmodulin-Like Proteins: A Biochemical and Structural Perspective.International journal of molecular sciences · 2018Review
- Preferential Binding of MgFrontiers in molecular neuroscience · 2018Article
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- Determination of Hydrodynamic Radius of Proteins by Size Exclusion Chromatography.Bio-protocol · 2017Article
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6 authors at 1 institution in 1 country.
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Abstract
In addition to the well-known Ca(2+) sensor calmodulin, plants possess many calmodulin-like proteins (CMLs) that are predicted to have specific roles in the cell. Herein, we described the biochemical and biophysical characterization of recombinant Arabidopsis thaliana CML14. We applied isothermal titration calorimetry to analyze the energetics of Ca(2+) and Mg(2+) binding to CML14, and nuclear magnetic resonance spectroscopy, together with intrinsic and ANS-based fluorescence, to evaluate the structural effects of metal binding and metal-induced conformational changes. Furthermore, differential scanning calorimetry and limited proteolysis were used to characterize protein thermal and local stability. Our data demonstrate that CML14 binds one Ca(2+) ion with micromolar affinity (Kd ∼ 12 µM) and the presence of 10 mM Mg(2+) decreases the Ca(2+) affinity by ∼5-fold. Although binding of Ca(2+) to CML14 increases protein stability, it does not result in a more hydrophobic protein surface and does not induce the large conformational rearrangement typical of Ca(2+) sensors, but causes only localized structural changes in the unique functional EF-hand. Our data, together with a molecular modelling prediction, provide interesting insights into the biochemical properties of Arabidopsis CML14 and may be useful to direct additional studies aimed at understanding its physiological role.
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