Evidence map›Paper›PMID 27124374›Full record

ArticleBiotechnic & histochemistry : official publication of the Biological Stain Commission2016

Refinements of and commentary on the silver staining techniques of Fernández-Galiano.

K J Aufderheide

Abstract read
In one paragraph

Article in Biotechnic & histochemistry : official publication of the Biological Stain Commission, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

K J Aufderheidea Department of Biology , Texas A&M University , College Station , Texas.

Funding

WELL-BEING AND UTILITY IN PSYCHOLOGY AND ECONOMICSP01AG026571 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WILLIS, ROBERT J · 2005 to 2014
$9.9M
GENETIC ANALYSIS OF TRICHOCYST DEVELOPMENT IN PARAMECIUMR01GM034681 · NIGMS · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI AUFDERHEIDE, KARL J · 1985 to 1987
–
NIA NIH HHS P01 AG026571NIA NIH HHS R01 AG002657NIGMS NIH HHS R01 GM034681
6 · The paper itself

Abstract

The original ammoniacal silver carbonate staining technique and subsequent modification developed by Fernández-Galiano are useful for investigating ciliate protozoan systematics and/or ciliate cortical structure and morphogenesis. The technique is complicated, however, by both uncertainties arising from the need to count drops of reagents and subjective control of the staining intensity. I have resolved these complications by defining volumes of reagents rather than using drops and by defining a range of staining times. I also comment on various steps of the techniques. My techniques are simplified and refined to produce consistent, high quality staining results.

Indexed as

Silver StainingHumansIndicators and ReagentsMicroscopyParameciumSilverammoniacal silverIndicators and ReagentsSilverciliatescortical structuresFernández-Galiano staining, Parameciumprotozoasilver staining

Identifiers

PMID27124374
PMCPMC5113713

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.