ArticlePloS one2016
The Nicotine-Evoked Locomotor Response: A Behavioral Paradigm for Toxicity Screening in Zebrafish (Danio rerio) Embryos and Eleutheroembryos Exposed to Methylmercury.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Zebrafish as an alternative method for determining the embryo toxicity of plant products: a systematic review.Environmental science and pollution research international · 2018Pooled it
- Prenatal Cannabis and Tobacco: Studies in Animal Models.Advances in experimental medicine and biology · 2026Review
- Short- and Long-Term Neurobehavioral Effects of Developmental Exposure to Valproic Acid in Zebrafish.International journal of molecular sciences · 2024Article
- Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model.International journal of molecular sciences · 2023Article
- Optimizing assays of zebrafish larvae swimming performance for drug discovery.Expert opinion on drug discovery · 2023Review
- Embryonic Nicotine Exposure Disrupts Adult Social Behavior and Craniofacial Development in Zebrafish.Toxics · 2022Article
- A Mini-Review Regarding the Modalities to Study Neurodevelopmental Disorders-Like Impairments in Zebrafish-Focussing on Neurobehavioural and Psychological Responses.Brain sciences · 2022Review
- Exploring Neurobehaviour in Zebrafish Embryos as a Screening Model for Addictiveness of Substances.Toxics · 2021Article
- Zebrafish Larvae as a Behavioral Model in Neuropharmacology.Biomedicines · 2019Review
- Developmental Methylmercury Exposure Affects Swimming Behavior and Foraging Efficiency of Yellow Perch (ACS omega · 2017Article
- Mercury-induced epigenetic transgenerational inheritance of abnormal neurobehavior is correlated with sperm epimutations in zebrafish.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
This study is an adaptation of the nicotine-evoked locomotor response (NLR) assay, which was originally utilized for phenotype-based neurotoxicity screening in zebrafish embryos. Zebrafish embryos do not exhibit spontaneous swimming until roughly 4 days post-fertilization (dpf), however, a robust swimming response can be induced as early as 36 hours post-fertilization (hpf) by means of acute nicotine exposure (30-240μM). Here, the NLR was tested as a tool for early detection of locomotor phenotypes in 36, 48 and 72 hpf mutant zebrafish embryos of the non-touch-responsive maco strain; this assay successfully discriminated mutant embryos from their non-mutant siblings. Then, methylmercury (MeHg) was used as a proof-of-concept neurotoxicant to test the effectiveness of the NLR assay as a screening tool in toxicology. The locomotor effects of MeHg were evaluated in 6 dpf wild type eleutheroembryos exposed to waterborne MeHg (0, 0.01, 0.03 and 0.1μM). Afterwards, the NLR assay was tested in 48 hpf embryos subjected to the same MeHg exposure regimes. Embryos exposed to 0.01 and 0.03μM of MeHg exhibited significant increases in locomotion in both scenarios. These findings suggest that similar locomotor phenotypes observed in free swimming fish can be detected as early as 48 hpf, when locomotion is induced with nicotine.
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Registered trials
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